Synthesis and biological evaluation of novel alkyl amide functionalized trifluoromethyl substituted pyrazolo[3,4-b]pyridine derivatives as potential anticancer agents

A series of novel alkyl amide functionalized trifluoromethyl substituted pyrazolo[3,4-b]pyridine derivatives 5, 6 and 7 were prepared starting from 6-phenyl-4-(trifluoromethyl)-1H-pyrazolo[3,4-b]pyridin-3-amine 3 via selective N-alkylation, followed by reaction with different primary aliphatic amines, cyclic secondary amines or l-amino acids under different set of conditions. All the synthesized compounds 5, 6 and 7 were screened for Anticancer activity against four Cancer cell lines such as A549-Lung Cancer (CCL-185), MCF7-Breast Cancer (HTB-22), DU145-Prostate Cancer (HTB-81) and HeLa-Cervical Cancer (CCL-2). The compounds 5i and 6e are found to have promising bioactivity at micro molar concentration.

Alkylation; Anticancer activity; Cyclic secondary amines; Primary aliphatic amines; Pyrazolo[3,4-b]pyridine; l-Amino acids.