2. Academic Validation
  3. Purification of antibiotics from the biocontrol agent Streptomyces anulatus S37 by centrifugal partition chromatography

Purification of antibiotics from the biocontrol agent Streptomyces anulatus S37 by centrifugal partition chromatography

  • J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 1:944:30-4. doi: 10.1016/j.jchromb.2013.11.008.
Olivier Couillerot 1,  Souad Loqman 2,  Alix Toribio 3,  Jane Hubert 4,  Léa Gandner 1,  Jean-Marc Nuzillard 3,  Yedir Ouhdouch 5,  Christophe Clément 1,  Essaid Ait Barka 1,  Jean-Hugues Renault 3
Affiliations

Affiliations

  • 1 Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne, UFR Sciences exactes et naturelles - UPRES EA 4707, Université de Reims Champagne-Ardenne, Bât. 18, Moulin de la Housse, BP 1039, 51687 Reims Cedex 2, France.
  • 2 Faculté des Sciences de Semlalia, Université Cadi Ayyad (UCAM), Laboratoire de Biologie et de Biotechnologie des Microorganismes, Marrakech, Morocco; UMR CNRS 7312, Institut de Chimie Moléculaire de Reims, Université de Reims Champagne-Ardenne, Bât. 18, Moulin de la Housse, BP 1039, 51687 Reims Cedex 2, France; Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne, UFR Sciences exactes et naturelles - UPRES EA 4707, Université de Reims Champagne-Ardenne, Bât. 18, Moulin de la Housse, BP 1039, 51687 Reims Cedex 2, France.
  • 3 UMR CNRS 7312, Institut de Chimie Moléculaire de Reims, Université de Reims Champagne-Ardenne, Bât. 18, Moulin de la Housse, BP 1039, 51687 Reims Cedex 2, France.
  • 4 UMR CNRS 7312, Institut de Chimie Moléculaire de Reims, Université de Reims Champagne-Ardenne, Bât. 18, Moulin de la Housse, BP 1039, 51687 Reims Cedex 2, France. Electronic address: jane.hubert@univ-reims.fr.
  • 5 Faculté des Sciences de Semlalia, Université Cadi Ayyad (UCAM), Laboratoire de Biologie et de Biotechnologie des Microorganismes, Marrakech, Morocco.
PMID: 24291716 DOI: 10.1016/j.jchromb.2013.11.008
Abstract

A novel actinomycete strain, Streptomyces anulatus S37, has been isolated from the rhizosphere of healthy Moroccan Vitis vinifera on the basis on its ability to promote grapevine growth and to induce natural defences against various phytopathogens. In the present work, the main bioactive metabolites produced by S. anulatus S37 were isolated. A crude n-BuOH extract of the S37 fermentation broth was firstly partitioned in a biphasic solvent system composed of n-heptane, methanol, and water (5:1.5:3.5, v/v). The most active organic fraction (1.1g) as revealed by TLC-bioautography was subsequently separated by a two-step centrifugal partition chromatography procedure. The first separation was performed in the ascending mode at 6mL/min with the biphasic solvent system n-heptane, ethyl acetate, methanol and water (2:1:2:1, v/v), to finally recover 40mg of a pure compound identified as streptochlorin by NMR spectroscopy. In a second separation, the solvent system n-heptane, acetonitrile, and water (5:5:4, v/v) was used in the ascending mode at 3mL/min to purify 135mg of nigericin and 53mg of piericidin A1. Assays performed with the three compounds have confirmed their inhibitory impact on the growth of Botryris cinerea in dual confrontation and also on V. vinifera L. plantlets.

Keywords

Botrytis cinerea; Centrifugal partition chromatography; Streptomyces; Vitis vinifera.

Figures
Products