2. Academic Validation
  3. New anti-inflammatory cembranoid diterpenoids from the Vietnamese soft coral Lobophytum crassum

New anti-inflammatory cembranoid diterpenoids from the Vietnamese soft coral Lobophytum crassum

  • Bioorg Med Chem Lett. 2014 Jan 1;24(1):228-32. doi: 10.1016/j.bmcl.2013.11.033.
Nguyen Phuong Thao 1,  Bui Thi Thuy Luyen 1,  Nguyen Thi Thanh Ngan 2,  Seok Bean Song 2,  Nguyen Xuan Cuong 3,  Nguyen Hoai Nam 3,  Phan Van Kiem 3,  Young Ho Kim 4,  Chau Van Minh 5
Affiliations

Affiliations

  • 1 Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Viet Nam; College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
  • 2 College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
  • 3 Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Viet Nam.
  • 4 College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: yhk@cnu.ac.kr.
  • 5 Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Viet Nam. Electronic address: cvminh@vast.ac.vn.
PMID: 24314396 DOI: 10.1016/j.bmcl.2013.11.033
Abstract

Four new cembranoid diterpenes lobocrasols A-D (1-4), were isolated from the methanol extract of the soft coral Lobophytum crassum. Their structures were elucidated by spectroscopic analysis and by comparison of the spectroscopic data with those of similar compounds previously reported in literature. The anti-inflammatory effects of isolated compounds were evaluated using NF-κB luciferase and reverse transcription polymerase chain reaction (RT-PCR). Compounds 1 and 2 significantly inhibited TNFα-induced NF-κB transcriptional activity in HepG2 cells in a dose-dependent manner, with IC50 values of 6.30±0.42 and 6.63±0.11μM, respectively. Furthermore, the transcriptional inhibition of these compounds was confirmed by a decrease in cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression levels in HepG2 cells.

Keywords

COX-2; Cembranoid; Lobophytum crassum; Soft coral; anti-Inflammatory; iNOS.

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