2. Academic Validation
  3. DNA replication is the target for the antibacterial effects of nonsteroidal anti-inflammatory drugs

DNA replication is the target for the antibacterial effects of nonsteroidal anti-inflammatory drugs

  • Chem Biol. 2014 Apr 24;21(4):481-487. doi: 10.1016/j.chembiol.2014.02.009.
Zhou Yin 1,  Yao Wang 1,  Louise R Whittell 1,  Slobodan Jergic 1,  Michael Liu 2,  Elizabeth Harry 2,  Nicholas E Dixon 1,  Michael J Kelso 1,  Jennifer L Beck 1,  Aaron J Oakley 3
Affiliations

Affiliations

  • 1 School of Chemistry and Centre for Medical and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
  • 2 iThree institute, University of Technology, Sydney, NSW 2007, Australia.
  • 3 School of Chemistry and Centre for Medical and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia. Electronic address: aarono@uow.edu.au.
PMID: 24631121 DOI: 10.1016/j.chembiol.2014.02.009
Abstract

Evidence suggests that some nonsteroidal anti-inflammatory drugs (NSAIDs) possess Antibacterial properties with an unknown mechanism. We describe the in vitro Antibacterial properties of the NSAIDs carprofen, bromfenac, and vedaprofen, and show that these NSAIDs inhibit the Escherichia coli DNA Polymerase III β subunit, an essential interaction hub that acts as a mobile tether on DNA for many essential partner proteins in DNA replication and repair. Crystal structures show that the three NSAIDs bind to the sliding clamp at a common binding site required for partner binding. Inhibition of interaction of the clamp loader and/or the replicative polymerase α subunit with the sliding clamp is demonstrated using an in vitro DNA replication assay. NSAIDs thus present promising lead scaffolds for novel Antibacterial agents targeting the sliding clamp.

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