2. Academic Validation
  3. Anti-inflammatory components of Euphorbia humifusa Willd

Anti-inflammatory components of Euphorbia humifusa Willd

  • Bioorg Med Chem Lett. 2014 Apr 15;24(8):1895-900. doi: 10.1016/j.bmcl.2014.03.014.
Bui Thi Thuy Luyen 1,  Bui Huu Tai 2,  Nguyen Phuong Thao 2,  Kim Ji Eun 1,  Ji Yun Cha 3,  Ming Jie Xin 3,  Young Mi Lee 4,  Young Ho Kim 5
Affiliations

Affiliations

  • 1 College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
  • 2 College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea; Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
  • 3 Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Iksan, Jeonbuk 570-749, Republic of Korea.
  • 4 Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Iksan, Jeonbuk 570-749, Republic of Korea. Electronic address: ymlee@wku.ac.kr.
  • 5 College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: yhk@cnu.ac.kr.
PMID: 24679441 DOI: 10.1016/j.bmcl.2014.03.014
Abstract

Two new compounds, euphorbinoside (1) and dehydropicrorhiza acid methyl diester (2), along with 24 known compounds (3-26) were isolated from Euphorbia humifusa Willd. The effects of these compounds on soluble Epoxide Hydrolase (sEH) inhibitory activity were evaluated. Flavonoid compounds (10-21) exhibited high sEH inhibitory activity. Among them, compounds 12, 13, and 19 greatly inhibited sEH enzymatic activity, with IC50 values as low as 18.05±1.17, 18.64±1.83, and 17.23±0.84 μM, respectively. In addition, the effects of these compounds on lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis factor alpha (TNF-α) production by RAW 264.7 cells were investigated. Compounds 3-6, 8, 18, 20-23, and 25-26 inhibited the production of both NO and TNF-α, with IC50 values ranging from 11.1±0.9 to 45.3±1.6 μM and 14.4±0.5 to 44.5±1.2 μM, respectively.

Keywords

Dehydropicrorhiza acid methyl diester; Euphorbia humifusa; Euphorbinoside; NO; Soluble epoxide hydrolase; TNF-α.

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