2. Academic Validation
  3. Cytotoxic constituents from Lobaria scrobiculata and a comparison of two bioassays for their evaluation

Cytotoxic constituents from Lobaria scrobiculata and a comparison of two bioassays for their evaluation

  • J Nat Prod. 2014 Apr 25;77(4):1069-73. doi: 10.1021/np4008574.
Andreas Schinkovitz 1,  Aman Kaur,  Ernst Urban,  Martin Zehl,  Gabriela Páchniková,  Yuehong Wang,  Nadine Kretschmer,  Iva Slaninová,  Guido F Pauli,  Scott G Franzblau,  Georg Krupitza,  Rudolf Bauer,  Brigitte Kopp
Affiliations

Affiliation

  • 1 Department of Pharmacognosy, Faculty of Life Sciences, University of Vienna , Althanstraße 14, A-1090 Vienna, Austria.
PMID: 24725159 DOI: 10.1021/np4008574
Abstract

Lichens are resilient organisms, known for their unique profiles of secondary metabolites and for exhibiting antioxidative, Antibacterial, and cytotoxic effects. Analyzing the cytotoxic potential of Lobaria scrobiculata, a bioassay-guided fractionation strategy yielded seven known metabolites, with two of these compounds, 2 and 3, exhibiting cytotoxicity against HL-60 cells. In order to verify the potential impact of degradation on observed bioactivity, a purity and stability evaluation was conducted. The consistency of results obtained by the water-soluble tetrazolium salt-1 assay and trypan blue cytotoxicity assay was evaluated for selected compounds.

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