2. Academic Validation
  3. DMU-212 inhibits tumor growth in xenograft model of human ovarian cancer

DMU-212 inhibits tumor growth in xenograft model of human ovarian cancer

  • Biomed Pharmacother. 2014 May;68(4):397-400. doi: 10.1016/j.biopha.2014.02.001.
Hanna Piotrowska 1,  Krzysztof Myszkowski 2,  Joanna Abraszek 3,  Eliza Kwiatkowska-Borowczyk 4,  Ryszard Amarowicz 5,  Marek Murias 6,  Marcin Wierzchowski 7,  Jadwiga Jodynis-Liebert 8
Affiliations

Affiliations

  • 1 Department of Toxicology, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: hanna.piotrowska@ump.edu.pl.
  • 2 Department of Toxicology, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: myszkowski.krzysztof.a@gmail.com.
  • 3 Department of Toxicology, Poznan University of Medical Sciences, Poznan, Poland.
  • 4 Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan, Poland; Department of Cancer Diagnostics and Immunology, Greater Poland Cancer Centre, Poznan, Poland. Electronic address: eliza.kwiatkowska@wco.pl.
  • 5 Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland. Electronic address: amaro@pan.olsztyn.pl.
  • 6 Department of Toxicology, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: marek.murias@ump.edu.pl.
  • 7 Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: mwierzch@ump.edu.pl.
  • 8 Department of Toxicology, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: liebert@ump.edu.pl.
PMID: 24768110 DOI: 10.1016/j.biopha.2014.02.001
Abstract

DMU-212 has been shown to evoke a mitochondrial apoptotic pathway in transformed fibroblasts and breast Cancer. However, recently published data indicated the ability of DMU-212 to evoke Apoptosis in both mitochondria- and receptor-mediated manner in two ovarian Cancer cell lines, namely A-2780 and SKOV-3, which showed varied sensitivity to the compound tested. The pronounced cytotoxic effects of DMU-212 observed in A-2780 cells were related to the execution of extracellular Apoptosis pathway and cell cycle arrest in G2/M phase. In view of the great Anticancer potential of DMU-212 against A-2780 cell line, the aim of the current study was to assess antiproliferative activity of DMU-212 in xenograft model of ovarian Cancer. To evaluate in vitro metabolic properties of cells that were to be injected into SCID mice, uptake and decline of DMU-212 in A-2780 ovarian Cancer cell line was investigated. It was found that the concentration of the test compound in A-2780 cells was growing within first eight hours, and then the gradual decline was observed. A-2780 cells stably transfected with pcDNA3.1/Zeo(-)-Luc vector were subcutaneously inoculated into the right flanks of SCID mice. After seven days of the treatment with DMU-212 (50mg/kg b.w), tumor growth appeared to be suppressed in the Animals treated with the compound tested. At day 14 of the experiment, tumor burden in mice treated with DMU-212 was significantly lower, as compared to untreated controls. Our findings suggest that DMU-212 might be considered as a potential Anticancer agent used in ovarian Cancer therapy.

Keywords

3,4,4′5-tetramethoxystilbene (DMU-212); Ovarian cancer; Xenograft model.

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