2. Academic Validation
  3. Alpha-phellandrene-induced DNA damage and affect DNA repair protein expression in WEHI-3 murine leukemia cells in vitro

Alpha-phellandrene-induced DNA damage and affect DNA repair protein expression in WEHI-3 murine leukemia cells in vitro

  • Environ Toxicol. 2015 Nov;30(11):1322-30. doi: 10.1002/tox.22003.
Jen-Jyh Lin 1 2,  Chih-Chung Wu 3,  Shu-Chun Hsu 4,  Shu-Wen Weng 1 5,  Yi-Shih Ma 6 7,  Yi-Ping Huang 8,  Jaung-Geng Lin 1,  Jing-Gung Chung 4 9
Affiliations

Affiliations

  • 1 Graduate Institute of Chinese Medicine, China Medical University, Taichung, 404, Taiwan.
  • 2 Division of Cardiology, China Medical University Hospital, Taichung, 404, Taiwan.
  • 3 Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan, 711, Taiwan.
  • 4 Department of Biological Science and Technology, China Medical University, Taichung, 404, Taiwan.
  • 5 Department of Chinese Medicine, Taichung Hospital, Department of Health, Executive Yuan, Taichung, 403, Taiwan.
  • 6 School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung, 84001, Taiwan.
  • 7 Department of Chinese Medicine, E-Da Hospital, Kaohsiung, 82445, Taiwan.
  • 8 Department of Physiology, China Medical University, Taichung, 404, Taiwan.
  • 9 Department of Biotechnology, Asia University, Taichung, 413, Taiwan, R.O.C.
PMID: 24861204 DOI: 10.1002/tox.22003
Abstract

Although there are few reports regarding α-phellandrene (α-PA), a natural compound from Schinus molle L. essential oil, there is no report to show that α-PA induced DNA damage and affected DNA repair associated protein expression. Herein, we investigated the effects of α-PA on DNA damage and repair associated protein expression in murine leukemia cells. Flow cytometric assay was used to measure the effects of α-PA on total cell viability and the results indicated that α-PA induced cell death. Comet assay and 4,6-diamidino-2-phenylindole dihydrochloride staining were used for measuring DNA damage and condensation, respectively, and the results indicated that α-PA induced DNA damage and condensation in a concentration-dependent manner. DNA gel electrophoresis was used to examine the DNA damage and the results showed that α-PA induced DNA damage in WEHI-3 cells. Western blotting assay was used to measure the changes of DNA damage and repair associated protein expression and the results indicated that α-PA increased p-p53, p-H2A.X, 14-3-3-σ, and MDC1 protein expression but inhibited the protein of p53, MGMT, DNA-PK, and BRCA-1.

Keywords

DNA damage and repair; WEHI-3 leukemia cells; α-phellandrene.

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