2. Academic Validation
  3. TRF2, but not TBP, mediates the transcription of ribosomal protein genes

TRF2, but not TBP, mediates the transcription of ribosomal protein genes

  • Genes Dev. 2014 Jul 15;28(14):1550-5. doi: 10.1101/gad.245662.114.
Yuan-Liang Wang 1,  Sascha H C Duttke 1,  Kai Chen 2,  Jeff Johnston 2,  George A Kassavetis 1,  Julia Zeitlinger 3,  James T Kadonaga 1
Affiliations

Affiliations

  • 1 Section of Molecular Biology, University of California at San Diego, La Jolla, California 92093, USA;
  • 2 Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA;
  • 3 Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA; Department of Pathology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.
PMID: 24958592 DOI: 10.1101/gad.245662.114
Abstract

The TCT core promoter element is present in most ribosomal protein (RP) genes in Drosophila and humans. Here we show that TBP (TATA box-binding protein)-related factor TRF2, but not TBP, is required for transcription of the TCT-dependent RP genes. In cells, TCT-dependent transcription, but not TATA-dependent transcription, increases or decreases upon overexpression or depletion of TRF2. In vitro, purified TRF2 activates TCT but not TATA promoters. ChIP-seq (chromatin immunoprecipitation [ChIP] combined with deep Sequencing) experiments revealed the preferential localization of TRF2 at TCT versus TATA promoters. Hence, a specialized TRF2-based RNA polymerase II system functions in the synthesis of RPs and complements the RNA polymerase I and III systems.

Keywords

RNA polymerase II; TCT motif; TRF2; core promoter; ribosomal protein genes.

Figures