2. Academic Validation
  3. MiR-221 accentuates IFN׳s anti-HCV effect by downregulating SOCS1 and SOCS3

MiR-221 accentuates IFN׳s anti-HCV effect by downregulating SOCS1 and SOCS3

  • Virology. 2014 Aug:462-463:343-50. doi: 10.1016/j.virol.2014.06.024.
Gang Xu 1,  Fang Yang 2,  Cui-Ling Ding 1,  Jing Wang 3,  Ping Zhao 1,  Wen Wang 4,  Hao Ren 5
Affiliations

Affiliations

  • 1 Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai 200433, China.
  • 2 Department of Hepatobiliary Surgery, Fuzhou General Hospital of Nanjing Military Area Command, Fuzhou 350025, China.
  • 3 Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
  • 4 Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai 200433, China. Electronic address: wonwin29@126.com.
  • 5 Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai 200433, China. Electronic address: hmren@yahoo.com.
PMID: 25019494 DOI: 10.1016/j.virol.2014.06.024
Abstract

MiR-221 was reported to be upregulated and play roles in tumorigenesis of hepatitis C virus (HCV) associated hepatocellular carcinoma (HCC). However, the role of miR-221 in HCV Infection remains unknown. In this study, it was found that miR-221 was upregulated in serum of HCV chronic hepatitis patients and Huh7.5.1 cells infected with HCVcc. Further studies indicated that miR-221 mimic could accentuate anti-HCV effect of IFN-α in HCVcc model, miR-221 mimic could further repressed 10% HCV RNA expression and 35-42% HCV core or NS5A protein expression in HCVcc infected Huh7.5.1 cells treated with 100IU/mL IFN-α, and miR-221 inhibitor resulted in the reverse effects. Furthermore, two members of suppressor of cytokine signaling (SOCS) family, SOCS1 and SOCS3, which are well established inhibitory factors on IFN/JAK/STAT pathway, were identified as the targets of miR-221 and were involved in the effect of miR-221. In conclusion, miR-221 could accentuate IFN׳s anti-HCV effect by targeting SOCS1 and SOCS3.

Keywords

HCV; IFN-α; Mir-221; SOCS1; SOCS3.

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