2. Academic Validation
  3. Novel dammarane saponins from Gynostemma pentaphyllum and their cytotoxic activities against HepG2 cells

Novel dammarane saponins from Gynostemma pentaphyllum and their cytotoxic activities against HepG2 cells

  • Bioorg Med Chem Lett. 2014 Oct 15;24(20):4831-3. doi: 10.1016/j.bmcl.2014.08.059.
Xiang-Lan Piao 1,  Shao-Fang Xing 2,  Cai-Xia Lou 3,  Dao-Jin Chen 2
Affiliations

Affiliations

  • 1 Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China. Electronic address: xlpiao@163.com.
  • 2 Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China.
  • 3 Laboratory of Comparative Medicine, Guangdong Medical Laboratory Animal Center, Foshan, Guangdong 528248, China.
PMID: 25227718 DOI: 10.1016/j.bmcl.2014.08.059
Abstract

Two new dammarane saponins, 2α,3β,12β-trihydroxydammar-20(22),24-diene-3-O-[β-D-glucopyranoxyl(1→2)-β-D-6″-O-acetylglucopyranoside (1, namely damulin C) and 2α,3β,12β-trihydroxydammar-20(21),24-diene-3-O-[β-D-glucopyranoxyl(1→2)-β-D-6″-O-acetylglucopyranoside (2, namely damulin D), were isolated from the ethanol extract of Gynostemma pentaphyllum, which had been heat processed by steaming at 125 °C. The NMR spectroscopic data of the novel saponins were completely assigned by using a combination of 2D NMR experiments including (1)H-(1)H COSY, HSQC, and HMBC. Their cytotoxic activities of human liver adenocarcinoma HepG2 cells were evaluated in vitro. They showed cytotoxicities against HepG2 cell line with IC50 of 40±0.7 and 38±0.5 μg/ml, respectively.

Keywords

Dammarane saponin; Damulin C; Damulin D; Gynostemma pentaphyllum; HepG2 cell cytotoxic activity.

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