2. Academic Validation
  3. CSBF/C10orf99, a novel potential cytokine, inhibits colon cancer cell growth through inducing G1 arrest

CSBF/C10orf99, a novel potential cytokine, inhibits colon cancer cell growth through inducing G1 arrest

  • Sci Rep. 2014 Oct 29;4:6812. doi: 10.1038/srep06812.
Wen Pan 1,  Yingying Cheng 1,  Heyu Zhang 2,  Baocai Liu 1,  Xiaoning Mo 1,  Ting Li 1,  Lin Li 3,  Xiaojing Cheng 3,  Lianhai Zhang 3,  Jiafu Ji 3,  Pingzhang Wang 1,  Wenling Han 1
Affiliations

Affiliations

  • 1 Department of Immunology, Center for Human Disease Genomics, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China.
  • 2 1] Department of Immunology, Center for Human Disease Genomics, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China [2] Central Laboratory, Peking University School of Stomatology, Beijing, China.
  • 3 1] Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing, China [2] Department of Surgery, Peking University Cancer Hospital &Institute, Beijing, China.
PMID: 25351403 DOI: 10.1038/srep06812
Abstract

Cytokines are soluble proteins that exert their functions by binding specific receptors. Many cytokines play essential roles in carcinogenesis and have been developed for the treatment of Cancer. In this study, we identified a novel potential cytokine using immunogenomics designated colon-derived SUSD2 binding factor (CSBF), also known as chromosome 10 open reading frame 99 (C10orf99). CSBF/C10orf99 is a classical secreted protein with predicted molecular mass of 6.5 kDa, and a functional ligand of Sushi Domain Containing 2 (SUSD2). CSBF/C10orf99 has the highest expression level in colon tissue. Both CSBF/C10orf99 and SUSD2 are down-regulated in colon Cancer tissues and cell lines with different regulation mechanisms. CSBF/C10orf99 interacts with SUSD2 to inhibit colon Cancer cell growth and induce G1 cell cycle arrest by down-regulating cyclin D and cyclin-dependent kinase 6 (CDK6). CSBF/C10orf99 displays a bell-shaped activity curve with the optimal effect at ~10 ng/ml. Its growth inhibitory effects can be blocked by sSUSD2-Fc soluble protein. Our results suggest that CSBF/C10orf99 is a novel potential cytokine with tumor suppressor functions.

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