2. Academic Validation
  3. The synergistic antitumor effects of all-trans retinoic acid and C-phycocyanin on the lung cancer A549 cells in vitro and in vivo

The synergistic antitumor effects of all-trans retinoic acid and C-phycocyanin on the lung cancer A549 cells in vitro and in vivo

  • Eur J Pharmacol. 2015 Feb 15;749:107-14. doi: 10.1016/j.ejphar.2015.01.009.
Bing Li 1,  Mei-Hua Gao 2,  Xian-Ming Chu 3,  Lei Teng 4,  Cong-Yi Lv 5,  Peng Yang 6,  Qi-Feng Yin 7
Affiliations

Affiliations

  • 1 Department of Biology, Medical College of Qingdao University, Qingdao 266021, China. Electronic address: libing_516619@163.com.
  • 2 Department of Immunology, Medical College of Qingdao University, Qingdao 266021, China. Electronic address: meihuagao66@163.com.
  • 3 Department of Cardiology, The Affiliated Hospital of Medical College of Qingdao University, Qingdao 266021, China. Electronic address: chuxianming@medmail.com.cn.
  • 4 Department of Biology, Medical College of Qingdao University, Qingdao 266021, China. Electronic address: qtlei@sina.com.
  • 5 Department of Biology, Medical College of Qingdao University, Qingdao 266021, China. Electronic address: congyilv@163.com.
  • 6 Department of Biology, Medical College of Qingdao University, Qingdao 266021, China. Electronic address: 289614208@qq.com.
  • 7 Department of Biology, Medical College of Qingdao University, Qingdao 266021, China. Electronic address: yin264823@126.com.
PMID: 25617793 DOI: 10.1016/j.ejphar.2015.01.009
Abstract

The Anticancer effects and mechanism of all-trans retinoic acid (ATRA), C-phycocyanin (C-PC) or ATRA+C-PC on the growth of A549 cells were studied in in vitro and in vivo experiments. The effects of C-PC and ATRA on the growth of A549 cells were determined. The expression of CDK-4 and Caspase-3, and the cellular Apoptosis levels were detected. The tumor model was established by subcutaneous injection of A549 cells to the left axilla of the NU/NU mice. The weights of tumor and the spleen were tested. The viabilities of T-cells and spleen cells, TNF levels, the expression of Bcl-2 protein and Cyclin D1 gene were examined. Results showed both C-PC and ATRA could inhibit the growth of tumor cells in vivo and in vitro. ATRA+C-PC cooperatively showed a higher antitumor activity. The dosage of ATRA was reduced when it was administered with C-PC together, and the toxicity was reduced as well. ATRA+C-PC could decrease CDK-4 but increase Caspase-3 protein expression level and induce cell Apoptosis. ATRA alone could lower the activities of T lymphocytes and spleen weights, but the combination with C-PC could effectively promote viability of T cells and spleen. C-PC+ATRA could up-regulate TNF, and down-regulate Bcl-2 and Cyclin D1 gene. The combination might inhibit tumor growth by inhibiting the progress of cell cycle, inducing cell Apoptosis and enhancing the body immunity.

Keywords

A-549 cancer cells; All-trans retinoic acid; Apoptosis; C-phycocyanin; Cell proliferation.

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