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  3. Cepharanthine induces apoptosis through reactive oxygen species and mitochondrial dysfunction in human non-small-cell lung cancer cells

Cepharanthine induces apoptosis through reactive oxygen species and mitochondrial dysfunction in human non-small-cell lung cancer cells

  • Biochem Biophys Res Commun. 2015 May 1;460(2):136-42. doi: 10.1016/j.bbrc.2015.02.131.
Peiyan Hua 1,  Mei Sun 2,  Guangxin Zhang 1,  Yifan Zhang 1,  Xin Tian 3,  Xin Li 4,  Ranji Cui 5,  Xingyi Zhang 6
Affiliations

Affiliations

  • 1 Department of Thoracic Surgery, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, 130041, China.
  • 2 Department of Pathology, The Second Hospital of Jilin University, Changchun, 130041, China.
  • 3 Department of Cardiology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, 130041, China.
  • 4 Department of Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, Changchun, 130041, China.
  • 5 Department of Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, Changchun, 130041, China. Electronic address: cuiranji@hotmail.com.
  • 6 Department of Thoracic Surgery, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, 130041, China. Electronic address: xingyizhang@live.com.
PMID: 25747710 DOI: 10.1016/j.bbrc.2015.02.131
Abstract

Cepharanthine is a medicinal plant-derived natural compound which possesses potent anti-cancer properties. However, there is little report about its effects on lung Cancer cells. In this study, we investigated the effects of cepharanthine on the cell viability and Apoptosis in human non-small-cell lung Cancer H1299 and A549 cells. It was found that cepharanthine inhibited the growth of H1299 and A549 cells in a dose-dependent manner which was associated with the generation of Reactive Oxygen Species(ROS) and the dissipation of mitochondrial membrane potential (Δψm). These effects were markedly abrogated when cells were pretreated with N-acetylcysteine (NAC), a specific ROS inhibitor, indicating that the apoptosis-inducing effect of cepharanthine in lung Cancer cells was mediated by ROS. In addition, cepharanthine triggered Apoptosis in non-small lung Cancer cells via the upregulation of Bax, downregulation of Bcl-2 and significant activation of Caspase-3 and PARP. These results provide the rationale for further research and preclinical investigation of cepharanthine's anti-tumor effect against human non-small-cell lung Cancer.

Keywords

A549 cells; Apoptosis; Cepharanthine; H1299 cells; Non-small-cell lung cancer; ROS.

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