2. Academic Validation
  3. Identification and metabolomic analysis of chemical modulators for lipid accumulation in Crypthecodinium cohnii

Identification and metabolomic analysis of chemical modulators for lipid accumulation in Crypthecodinium cohnii

  • Bioresour Technol. 2015 Sep;191:362-8. doi: 10.1016/j.biortech.2015.03.068.
Jinghan Li 1,  Xiangfeng Niu 1,  Guangsheng Pei 1,  Xiao Sui 1,  Xiaoqing Zhang 1,  Lei Chen 1,  Weiwen Zhang 2
Affiliations

Affiliations

  • 1 Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; SynBio Platform, Collaborative Innovation Center of Chemical Science & Engineering, Tianjin, PR China.
  • 2 Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; SynBio Platform, Collaborative Innovation Center of Chemical Science & Engineering, Tianjin, PR China. Electronic address: wwzhang8@tju.edu.cn.
PMID: 25818259 DOI: 10.1016/j.biortech.2015.03.068
Abstract

In the study, fourteen chemical modulators from five groups (i.e., Auxin, gibberellin, Cytokinin, signal transducer and amine) were evaluated for their effects on lipid accumulation in Crypthecodinium cohnii. The results showed that naphthoxyacetic acid (BNOA), 2-chlorodracylicacid, salicylic acid (SA), Abscisic acid (ABA) and ethanolamine (ETA), increased lipid accumulation in C. cohnii by 10.00-18.78%. In addition, the combined uses of the above chemicals showed that two combinations, 1.0mg/L SA & 152.7 mg/L ETA and 4.0mg/L BNOA & 152.7 mg/L ETA, increased lipid accumulation by 22.45% and 20.54%, respectively. Moreover, a targeted metabolomic approach was employed to decipher the possible mechanisms responsible for the increased lipid accumulation, and the results showed that the enhanced metabolism in glycolysis and TCA cycle as well as the decreased metabolism in PPP pathway could be important for the stimulatory roles of BNOA & ETA and SA & ETA on lipid accumulation in C. cohnii.

Keywords

C. cohnii; Chemical modulators; Lipid; Metabolomics.

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