2. Academic Validation
  3. DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex

DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex

  • Exp Cell Res. 2015 May 15;334(1):146-59. doi: 10.1016/j.yexcr.2015.03.017.
Markus Kellner 1,  Michaela Rohrmoser 1,  Ignasi Forné 2,  Kirsten Voss 1,  Kaspar Burger 1,  Bastian Mühl 1,  Anita Gruber-Eber 1,  Elisabeth Kremmer 3,  Axel Imhof 2,  Dirk Eick 4
Affiliations

Affiliations

  • 1 Department of Molecular Epigenetics, Helmholtz Center Munich, Center for Integrated Protein Science Munich (CIPSM), Marchioninistr. 25, Munich 81377, Germany.
  • 2 Adolf Butenandt Institute, Ludwig Maximilians University of Munich, Center for Integrated Protein Science Munich (CIPSM), Schillerstr. 44, Munich 80336, Germany.
  • 3 Institute of Molecular Immunology, Helmholtz Center Munich, Marchioninistr. 25, Munich 81377, Germany.
  • 4 Department of Molecular Epigenetics, Helmholtz Center Munich, Center for Integrated Protein Science Munich (CIPSM), Marchioninistr. 25, Munich 81377, Germany. Electronic address: eick@helmholtz-muenchen.de.
PMID: 25825154 DOI: 10.1016/j.yexcr.2015.03.017
Abstract

PeBoW, a trimeric complex consisting of pescadillo (Pes1), block of proliferation (Bop1), and the WD repeat protein 12 (WDR12), is essential for processing and maturation of mammalian 5.8S and 28S ribosomal RNAs. Applying a mass spectrometric analysis, we identified the DEAD-box helicase DDX27 as stably associated factor of the PeBoW-complex. DDX27 interacts with the PeBoW-complex via an evolutionary conserved F×F motif in the N-terminal domain and is recruited to the nucleolus via its basic C-terminal domain. This recruitment is RNA-dependent and occurs independently of the PeBoW-complex. Interestingly, knockdown of DDX27, but not of Pes1, induces the accumulation of an extended form of the primary 47S rRNA. We conclude that DDX27 can interact specifically with the Pes1 and Bop1 but fulfils critical function(s) for proper 3' end formation of 47S rRNA independently of the PeBoW-complex.

Keywords

DEAD-box helicase; F×F motif; Nucleolus; PeBoW-complex; rRNA processing.

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