2. Academic Validation
  3. Genetic Incorporation of ε-N-2-Hydroxyisobutyryl-lysine into Recombinant Histones

Genetic Incorporation of ε-N-2-Hydroxyisobutyryl-lysine into Recombinant Histones

  • ACS Chem Biol. 2015 Jul 17;10(7):1599-603. doi: 10.1021/cb501055h.
Han Xiao 1,  Weimin Xuan 1,  Sida Shao 1,  Tao Liu 1,  Peter G Schultz 1
Affiliations

Affiliation

  • 1 Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, United States.
PMID: 25909834 DOI: 10.1021/cb501055h
Abstract

Here, we report the evolution of an orthogonal amber suppressor pyrrolysyl-tRNA synthetase (PylRS)/tRNACUA(Pyl) pair that genetically encodes the post-translationally modified amino acid, ε-N-2-hydroxyisobutyryl-lysine (HibK), in bacteria and mammalian cells. HibK is a new type of histone MARK that is widely distributed in histone proteins. The ability to site-specifically incorporate HibK into proteins provides a useful tool to probe the biological function of this newly identified post-translational modification.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-157140
    Histone Mark