2. Academic Validation
  3. Qualitative and Quantitative Analysis of Lignan Constituents in Caulis Trachelospermi by HPLC-QTOF-MS and HPLC-UV

Qualitative and Quantitative Analysis of Lignan Constituents in Caulis Trachelospermi by HPLC-QTOF-MS and HPLC-UV

  • Molecules. 2015 May 5;20(5):8107-24. doi: 10.3390/molecules20058107.
Xiao-Ting Liu 1 2,  Xu-Guang Wang 3,  Yu Yang,  Rui Xu 4,  Fan-Hua Meng 5,  Neng-Jiang Yu 6,  Yi-Min Zhao 7
Affiliations

Affiliations

  • 1 Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. liuxiaoting_2013@163.com.
  • 2 School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. liuxiaoting_2013@163.com.
  • 3 Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. xuguang_wang521@sina.com.
  • 4 Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. beijingxurui@sina.com.
  • 5 Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. mengfh331@126.com.
  • 6 Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. ynj08@hotmail.com.
  • 7 Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. zhaoym@nic.bmi.ac.cn.
PMID: 25951006 DOI: 10.3390/molecules20058107
Abstract

A high-performance liquid chromatography coupled with quadrupole tandem time-of-flight mass (HPLC-QTOF-MS) and ultraviolet spectrometry (HPLC-UV) was established for simultaneous qualitative and quantitative analysis of the major chemical constituents in Caulis Trachelospermi, respectively. The analysis was performed on an Agilent Zorbax Eclipse Plus C18 column (4.6 mm×150 mm, 5 μm) using a binary gradient system of water and methanol, with ultraviolet absorption at 230 nm. Based on high-resolution ESI-MS/MS fragmentation behaviors of the Reference Standards, the characteristic cleavage patterns of lignano-9, 9'-lactones and lignano-8'-hydroxy-9, 9'-lactones were obtained. The results demonstrated that the characteristic fragmentation patterns are valuable for identifying and differentiating lignano-9,9'-lactones and lignano-8'-hydroxy-9,9'-lactones. As such, a total of 25 compounds in Caulis Trachelospermi were unambiguously or tentatively identified via comparisons with Reference Standards or literature. In addition, 14 dibenzylbutyrolatone Lignans were simultaneously quantified in Caulis Trachelospermi by HPLC-UV method. The method is suitable for the qualitative and quantitative analyses of dibenzylbutyrolatone Lignans in Caulis Trachelospermi.

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