2. Academic Validation
  3. Surface plasmon resonance: a versatile technique for biosensor applications

Surface plasmon resonance: a versatile technique for biosensor applications

  • Sensors (Basel). 2015 May 5;15(5):10481-510. doi: 10.3390/s150510481.
Hoang Hiep Nguyen 1 2,  Jeho Park 3 4,  Sebyung Kang 5,  Moonil Kim 6 7 8
Affiliations

Affiliations

  • 1 BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. hhiep86@kribb.re.kr.
  • 2 Department of Nanobiotechnology, Korea University of Science and Technology (UST), Daejeon 305-350, Korea. hhiep86@kribb.re.kr.
  • 3 BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. jayho88@kribb.re.kr.
  • 4 Department of Nanobiotechnology, Korea University of Science and Technology (UST), Daejeon 305-350, Korea. jayho88@kribb.re.kr.
  • 5 Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798, Korea. sabsab7@unist.ac.kr.
  • 6 BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. kimm@kribb.re.kr.
  • 7 Department of Nanobiotechnology, Korea University of Science and Technology (UST), Daejeon 305-350, Korea. kimm@kribb.re.kr.
  • 8 Department of Pathobiology, College of Veterinary Medicine Nursing & Allied Health (CVMNAH), Tuskegee University, Tuskegee, AL 36088, USA. kimm@kribb.re.kr.
PMID: 25951336 DOI: 10.3390/s150510481
Abstract

Surface plasmon resonance (SPR) is a label-free detection method which has emerged during the last two decades as a suitable and reliable platform in clinical analysis for biomolecular interactions. The technique makes it possible to measure interactions in real-time with high sensitivity and without the need of labels. This review article discusses a wide range of applications in optical-based sensors using either surface plasmon resonance (SPR) or surface plasmon resonance imaging (SPRI). Here we summarize the principles, provide examples, and illustrate the utility of SPR and SPRI through example applications from the biomedical, proteomics, genomics and bioengineering fields. In addition, SPR signal amplification strategies and surface functionalization are covered in the review.

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