2. Academic Validation
  3. Metabolites of arachidonic acid and linoleic acid in early stages of non-alcoholic fatty liver disease--A pilot study

Metabolites of arachidonic acid and linoleic acid in early stages of non-alcoholic fatty liver disease--A pilot study

  • Prostaglandins Other Lipid Mediat. 2015 Sep;121(Pt B):184-9. doi: 10.1016/j.prostaglandins.2015.09.003.
Dominika Maciejewska 1,  Piotr Ossowski 1,  Arleta Drozd 1,  Karina Ryterska 1,  Dominika Jamioł-Milc 1,  Marcin Banaszczak 1,  Małgorzata Kaczorowska 1,  Anna Sabinicz 1,  Joanna Raszeja-Wyszomirska 2,  Ewa Stachowska 3
Affiliations

Affiliations

  • 1 Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
  • 2 Department of General, Transplant and Surgery of the Obser, Warszawa, Poland.
  • 3 Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland. Electronic address: Polandewast@sci.pum.szczecin.pl.
PMID: 26408952 DOI: 10.1016/j.prostaglandins.2015.09.003
Abstract

Background: Nonalcoholic fatty liver disease (NAFLD) is a spectrum of liver conditions related to fat infiltration. The role of liver triacylglycerol accumulation in NAFLD is not fully understood.

Methods: Twenty-four patients, 12 in the first and 12 in the second stage of NAFLD, were prospectively enrolled in this study. Biochemical parameters and eicosanoids (HETE and HODE) were compared between the first and the second stage of hepatic steatosis and the effect of a 6-month dietary intervention on these parameters was evaluated. Eicosanoid profiles were extracted from 0.5 ml of plasma using solid-phase extraction RP-18 SPE columns. The HPLC separations were performed on a 1260 liquid chromatograph.

Results: Patients with stage I NAFLD had a significantly higher level of HDL Cholesterol and a lower level of 5-HETE. Patients with grade II steatosis had higher concentrations of 9-HODE. Following the six-month dietary intervention, hepatic steatosis resolved completely in all patients. This resulted in a significant decrease in the concentrations of all eicosanoids (LX4, 16-HETE, 13-HODE, 9-HODE, 15-HETE, 12-HETE, 5-oxoETE, 5-HETE) and key biochemical parameters (BMI, Insulin, HOMA-IR, liver Enzymes).

Conclusion: A significant reduction in the analyzed eicosanoids and a parallel reduction in fatty liver confirmed the usefulness of HETE and HODE in the assessment of NAFLD.

Keywords

Biochemical parameters; Eicosanoids; Inflammation; Inflammatory mediators; NAFLD.

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