2. Academic Validation
  3. (E)-4-(3,4-Dimethoxyphenyl)but-3-en-1-ol Enhances Melanogenesis through Increasing Upstream Stimulating Factor-1-Mediated Tyrosinase Expression

(E)-4-(3,4-Dimethoxyphenyl)but-3-en-1-ol Enhances Melanogenesis through Increasing Upstream Stimulating Factor-1-Mediated Tyrosinase Expression

  • PLoS One. 2015 Nov 4;10(11):e0141988. doi: 10.1371/journal.pone.0141988.
Jisu Park 1,  Heesung Chung 1,  Seung Hyun Bang 2,  Ah-Reum Han 3,  Eun-Kyoung Seo 3,  Sung Eun Chang 2,  Duk-Hee Kang 4,  Eok-Soo Oh 1
Affiliations

Affiliations

  • 1 Department of Life Sciences, the Research Center for Cellular Homeostasis, Ewha Womans University, Seoul, Korea.
  • 2 Department of Dermatology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
  • 3 The Global Top5 Research Program, College of Pharmacy, Ewha Womans University, Seoul, Korea.
  • 4 Division of Nephrology, Department of Internal Medicine, Ewha Medical Research Center, Ewha Womans University School of Medicine, Seoul, Korea.
PMID: 26535571 DOI: 10.1371/journal.pone.0141988
Abstract

We investigated the potential melanogenic effect of compounds from Zingiber cassumunar Roxb. Our data revealed that chloroform-soluble extract of Z. cassumunar enhanced melanin synthesis in B16F10 melanoma cells. Among the components of the chloroform extract, (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-ol (DMPB) increased melanogenesis in both B16F10 cells and human primary melanocytes. In B16F10 cells, DMPB enhanced the activation of ERK and p38, and the level of Tyrosinase. Although the level of microphthalmia-associated transcription factor was unchanged in DMPB-treated B16F10 cells, DMPB increased levels and nuclear localization of upstream stimulating factor-1 (USF1). Consistently, DMPB-mediated melanin synthesis was diminished in USF1-knockdown cells. Furthermore, DMPB induced hyperpigmentation in brown guinea pigs in vivo. Together, these data suggest that DMPB may promote melanin synthesis via USF1 dependent fashion and could be used as a clinical therapeutic agent against hypopigmentation-associated diseases.

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