2. Academic Validation
  3. The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals

The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals

  • Chem Commun (Camb). 2016 Jan 14;52(4):737-40. doi: 10.1039/c5cc07354e.
Hao-Ying Zhang 1,  Jun Xiong 1,  Bao-Ling Qi 1,  Yu-Qi Feng 1,  Bi-Feng Yuan 1
Affiliations

Affiliation

  • 1 Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. bfyuan@whu.edu.cn yqfeng@whu.edu.cn.
PMID: 26562407 DOI: 10.1039/c5cc07354e
Abstract

We developed a novel strategy by oxidation-derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.

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