2. Academic Validation
  3. Inhibitory Effect of Gardenoside on Free Fatty Acid-Induced Steatosis in HepG2 Hepatocytes

Inhibitory Effect of Gardenoside on Free Fatty Acid-Induced Steatosis in HepG2 Hepatocytes

  • Int J Mol Sci. 2015 Nov 20;16(11):27749-56. doi: 10.3390/ijms161126058.
Huiqing Liang 1 2,  Limin Zhang 3,  Hongguo Wang 4,  Jinmo Tang 5,  Jiaen Yang 6,  Chuncheng Wu 7,  Shaodong Chen 8
Affiliations

Affiliations

  • 1 Department of Traditional Chinese Medicine, Medical College of Xiamen University, 422 S. Siming RD, Xiamen 361005, China. 13850005898@163.com.
  • 2 Xiamen Hospital of Traditional Chinese Medicine, 1739 Xianyue RD, Xiamen 361009, China. 13850005898@163.com.
  • 3 Department of Traditional Chinese Medicine, Medical College of Xiamen University, 422 S. Siming RD, Xiamen 361005, China. 124520141153580@stu.xmu.edu.cn.
  • 4 Department of Traditional Chinese Medicine, Medical College of Xiamen University, 422 S. Siming RD, Xiamen 361005, China. 24520131153546@stu.xmu.edu.cn.
  • 5 Xiamen Hospital of Traditional Chinese Medicine, 1739 Xianyue RD, Xiamen 361009, China. 13850085858@163.com.
  • 6 Xiamen Hospital of Traditional Chinese Medicine, 1739 Xianyue RD, Xiamen 361009, China. yje1977@yeah.net.
  • 7 Xiamen Hospital of Traditional Chinese Medicine, 1739 Xianyue RD, Xiamen 361009, China. wccxmtcm@yeah.net.
  • 8 Department of Traditional Chinese Medicine, Medical College of Xiamen University, 422 S. Siming RD, Xiamen 361005, China. adong@xmu.edu.cn.
PMID: 26610473 DOI: 10.3390/ijms161126058
Abstract

Gardenoside is one of the most important effective extractions of a herb for its hepatoprotective properties. The aim of this study was to address the mechanism of Gardenoside on HepG2 cellular steatosis induced by free fatty acids (FFAs). The model of HepG2 steatosis was duplicated by oleic and palmitic acid at the proportion of 2:1 (FFAs mixture) for 24 h, then lipid toxicity was induced. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were used to detect cell viability and Oil Red O staining method was used to judge the lipid accumulation respectively. Inflammatory cytokines TNF-α, IL-1β, IL-6 and intracellular NFκB were measured after 24 h. The steatosis was significantly decreased after Gardenoside treatment without cytotoxicity. TNF-α, IL-1β, IL-6 were modulated to HepG2 cells by treatment of Gardenoside. In the meantime, the activation of NFκB was inhibited by Gardenoside. Gardenoside has a protective effect on FFA-induced cellular steatosis in HepG2 cells which indicates that Gardenoside might be a potential therapeutic herb against NASH by suppressed supernatant inflammatory cytokine production and intracellular NFkB activity.

Keywords

HepG2; NFκB; free fatty acid; gardenoside; inflammatory cytokines; steatosis.

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