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  3. A Multisite-Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells

A Multisite-Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells

  • Angew Chem Int Ed Engl. 2016 Jan 26;55(5):1773-6. doi: 10.1002/anie.201510003.
Lu Wang 1,  Lin Yuan 2 3,  Xian Zeng 4,  Juanjuan Peng 5,  Yong Ni 5,  Jun Cheng Er 1,  Wang Xu 1,  Bikram Keshari Agrawalla 1,  Dongdong Su 5,  Beomsue Kim 5,  Young-Tae Chang 6 7
Affiliations

Affiliations

  • 1 Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, 117543, Singapore.
  • 2 Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, 117543, Singapore. lyuan@hnu.edu.cn.
  • 3 State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 (PR, China. lyuan@hnu.edu.cn.
  • 4 Department of Pharmacy, National, University of Singapore, Singapore, 117543, Singapore.
  • 5 Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Singapore, 138667, Singapore.
  • 6 Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, 117543, Singapore. chmcyt@nus.edu.sg.
  • 7 Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Singapore, 138667, Singapore. chmcyt@nus.edu.sg.
PMID: 26676712 DOI: 10.1002/anie.201510003
Abstract

Adenosine triphosphate (ATP), commonly produced in mitochondria, is required by almost all the living organisms; thus fluorescent probes for monitoring mitochondrial ATP levels fluctuation are essential and highly desired. Herein, we report a multisite-binding switchable fluorescent probe, ATP-Red 1, which selectively and rapidly responds to intracellular concentrations of ATP. Live-cell imaging indicated that ATP-Red 1 mainly localized to mitochondria with good biocompatibility and membrane penetration. In particular, with the help of ATP-Red 1, we successfully observed not only the decreased mitochondrial ATP levels in the presence of KCN and starvation state, but also the increased mitochondrial ATP levels in the early stage of cell Apoptosis. These results indicate that ATP-Red 1 is a useful tool for investigating ATP-relevant biological processes.

Keywords

ATP; cell imaging; fluorescent probes; multisite-binding.

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