2. Academic Validation
  3. UbcD4, an ortholog of E2-25K/Ube2K, is essential for activation of the immune deficiency pathway in Drosophila

UbcD4, an ortholog of E2-25K/Ube2K, is essential for activation of the immune deficiency pathway in Drosophila

  • Biochem Biophys Res Commun. 2016 Jan 22;469(4):891-6. doi: 10.1016/j.bbrc.2015.12.062.
Eun Sil Park 1,  Muthukumar Elangovan 1,  Young-Joon Kim 1,  Yung Joon Yoo 2
Affiliations

Affiliations

  • 1 School of Life Sciences, Gwangju Institute of Science & Technology (GIST), Gwangju 500-712, South Korea.
  • 2 School of Life Sciences, Gwangju Institute of Science & Technology (GIST), Gwangju 500-712, South Korea. Electronic address: yjyoo@gist.ac.kr.
PMID: 26707646 DOI: 10.1016/j.bbrc.2015.12.062
Abstract

Ubiquitination is a key regulatory mechanism in the immune deficiency (IMD) pathway in Drosophila. In this study, we first developed a simple immunoblot method to identify components involved in this pathway. Considering the emerging roles of ubiquitin-conjugating Enzymes (E2s) in determining ubiquitin chain types and ubiquitination speed, we screened for E2s required for IMD activation. We found that UbcD4, in addition to the previously reported E2s Effete and Bendless, was required for activation of the IMD pathway. RNAi-mediated knockdown of the UbcD4 ortholog, E2-25K/Ube2K, inhibited TNFα- and LPS-mediated activation of the NF-κB pathway, implying that UbcD4 and E2-25K/Ube2K play a conserved role as positive regulators in both pathways.

Keywords

E2-25K/Ube2K; IMD pathway; NF-κB pathway; UbcD4; Ubiquitin-conjugating enzyme (E2).

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