2. Academic Validation
  3. Synthesis and biological evaluation of 5,10-dihydro-11H-dibenzo[b,e][1,4]diazepin-11-one structural derivatives as anti-cancer and apoptosis inducing agents

Synthesis and biological evaluation of 5,10-dihydro-11H-dibenzo[b,e][1,4]diazepin-11-one structural derivatives as anti-cancer and apoptosis inducing agents

  • Eur J Med Chem. 2016 Jan 27:108:674-686. doi: 10.1016/j.ejmech.2015.12.007.
Chintakunta Praveen Kumar 1,  T Srinivasa Reddy 2,  Prathama S Mainkar 3,  Vipul Bansal 4,  Ravi Shukla 2,  Srivari Chandrasekhar 5,  Helmut M Hügel 6
Affiliations

Affiliations

  • 1 School of Applied Sciences, RMIT University, Melbourne 3001, Australia; IICT-RMIT Research Centre SIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India; Division of Natural Products Chemistry, CSIR-IICT, Hyderabad 500 007, India.
  • 2 School of Applied Sciences, RMIT University, Melbourne 3001, Australia; Health Innovations Research Institute, RMIT University, Melbourne 3083, Australia; Ian Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory, School of Applied Sciences, RMIT University, Melbourne 3000, Australia; Centre for Advanced Materials and Industrial Chemistry, RMIT University, Melbourne 3000, Australia.
  • 3 Division of Natural Products Chemistry, CSIR-IICT, Hyderabad 500 007, India.
  • 4 School of Applied Sciences, RMIT University, Melbourne 3001, Australia; Health Innovations Research Institute, RMIT University, Melbourne 3083, Australia; Ian Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory, School of Applied Sciences, RMIT University, Melbourne 3000, Australia.
  • 5 Division of Natural Products Chemistry, CSIR-IICT, Hyderabad 500 007, India. Electronic address: srivaric@iict.res.in.
  • 6 School of Applied Sciences, RMIT University, Melbourne 3001, Australia. Electronic address: helmut.hugel@rmit.edu.au.
PMID: 26735909 DOI: 10.1016/j.ejmech.2015.12.007
Abstract

A series of thirteen 5H-dibenzo [b,e][1,4]diazepin-11(10H)-one structural derivatives has been synthesized and evaluated for anti-proliferative activity against five human Cancer cell lines. Compound 9a exhibited potent tumour growth inhibition in all cell lines with IC50 values in the range of 0.71-7.29 μM. Experiments on lung (A549) and breast (MDAMB-231) Cancer cell lines to investigate the mechanisms of growth inhibition and Apoptosis inducing effects of 9a showed that it arrested both Cancer cell lines in the G2/M phase of cell cycle in a dose dependent manner. Hoechst staining analysis revealed that 9a inhibited tumour cell proliferation through Apoptosis induction. Additionally, the mitochondrial membrane potential (ΔΨm) was affected and the levels of Reactive Oxygen Species (ROS) were raised. The simple synthetic preparation and their biological properties make these dibenzodiazepinone-triazole scaffolds promising new entities for the development of Cancer therapeutics.

Keywords

Anti-cancer activity; Apoptosis; Dibenzodiazepinones; Triazoles.

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