2. Academic Validation
  3. Functional Analysis of CP2-Like Domain and SAM-Like Domain in TFCP2L1, Novel Pluripotency Factor of Embryonic Stem Cells

Functional Analysis of CP2-Like Domain and SAM-Like Domain in TFCP2L1, Novel Pluripotency Factor of Embryonic Stem Cells

  • Appl Biochem Biotechnol. 2016 Jun;179(4):650-8. doi: 10.1007/s12010-016-2021-z.
Chang Min Kim 1,  Tae-Ho Jang 1,  Hyun Ho Park 2
Affiliations

Affiliations

  • 1 School of Chemistry and Biochemistry and Graduate School of Biochemistry, Yeungnam University, Gyeongsan, Republic of Korea.
  • 2 School of Chemistry and Biochemistry and Graduate School of Biochemistry, Yeungnam University, Gyeongsan, Republic of Korea. hyunho@ynu.ac.kr.
PMID: 26906118 DOI: 10.1007/s12010-016-2021-z
Abstract

TFCP2L1 is a transcription factor that facilitates establishment and maintenance of pluripotency in embryonic stem cells by forming a complex transcriptional network with Other transcription factors (OCT4, SOX2, and NANOG). TFCP2L1 contains two distinct domains, the CP2-like domain at the N-terminus and the SAM-like domain at the C-terminus. In this study, we found that TFCP2L1 is hexamerized in solution via the C-terminal SAM-like domain. We also found that homo-oligomerization of SAM-like domain is dependent on the concentration of the proteins. Finally, we found that TFCP2L1 binds directly to DNA via the N-terminal CP2-like domain.

Keywords

CP2 domain; Embryonic stem cell; Pluripotency factor; SAM domain; TFCP2L1.

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