2. Academic Validation
  3. MTAP deletion confers enhanced dependency on the PRMT5 arginine methyltransferase in cancer cells

MTAP deletion confers enhanced dependency on the PRMT5 arginine methyltransferase in cancer cells

  • Science. 2016 Mar 11;351(6278):1214-8. doi: 10.1126/science.aad5214.
Gregory V Kryukov 1,  Frederick H Wilson 1,  Jason R Ruth 1,  Joshiawa Paulk 1,  Aviad Tsherniak 2,  Sara E Marlow 1,  Francisca Vazquez 1,  Barbara A Weir 1,  Mark E Fitzgerald 2,  Minoru Tanaka 1,  Craig M Bielski 1,  Justin M Scott 2,  Courtney Dennis 2,  Glenn S Cowley 2,  Jesse S Boehm 2,  David E Root 2,  Todd R Golub 2,  Clary B Clish 2,  James E Bradner 1,  William C Hahn 1,  Levi A Garraway 3
Affiliations

Affiliations

  • 1 Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA. The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • 2 The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • 3 Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA. The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. levi_garraway@dfci.harvard.edu.
PMID: 26912360 DOI: 10.1126/science.aad5214
Abstract

The discovery of Cancer dependencies has the potential to inform therapeutic strategies and to identify putative drug targets. Integrating data from comprehensive genomic profiling of Cancer cell lines and from functional characterization of Cancer cell dependencies, we discovered that loss of the Enzyme methylthioadenosine phosphorylase (MTAP) confers a selective dependence on protein arginine methyltransferase 5 (PRMT5) and its binding partner WDR77. MTAP is frequently lost due to its proximity to the commonly deleted tumor suppressor gene, CDKN2A. We observed increased intracellular concentrations of methylthioadenosine (MTA, the metabolite cleaved by MTAP) in cells harboring MTAP deletions. Furthermore, MTA specifically inhibited PRMT5 enzymatic activity. Administration of either MTA or a small-molecule PRMT5 Inhibitor showed a modest preferential impairment of cell viability for MTAP-null Cancer cell lines compared with isogenic MTAP-expressing counterparts. Together, our findings reveal PRMT5 as a potential vulnerability across multiple Cancer lineages augmented by a common "passenger" genomic alteration.

Figures
Products