2. Academic Validation
  3. Antimalarial Oxoprotoberberine Alkaloids from the Leaves of Miliusa cuneata

Antimalarial Oxoprotoberberine Alkaloids from the Leaves of Miliusa cuneata

  • J Nat Prod. 2016 Apr 22;79(4):978-83. doi: 10.1021/acs.jnatprod.5b01054.
Thanika Promchai 1,  Atchara Jaidee 1,  Sarot Cheenpracha 2,  Kongkiat Trisuwan 1,  Roonglawan Rattanajak 3,  Sumalee Kamchonwongpaisan 3,  Surat Laphookhieo 4,  Stephen G Pyne 5,  Thunwadee Ritthiwigrom 1
Affiliations

Affiliations

  • 1 Department of Chemistry, Faculty of Science, Chiang Mai University , Sutep, Muang, Chiang Mai 50200, Thailand.
  • 2 School of Science, University of Phayao , Maeka, Muang, Phayao 56000, Thailand.
  • 3 Medical Molecular Biology Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency , Thailand Science Park, Pathumthani 12120, Thailand.
  • 4 Natural Products Research Laboratory, School of Science, Mae Fah Luang University , Tasud, Muang, Chiang Rai 57100, Thailand.
  • 5 School of Chemistry, University of Wollongong , Wollongong, New South Wales 2522, Australia.
PMID: 26928423 DOI: 10.1021/acs.jnatprod.5b01054
Abstract

Five new oxoprotoberberine Alkaloids, miliusacunines A-E (1-5), along with nine known compounds, 6-14, were isolated from an acetone extract of the leaves and twigs of Miliusa cuneata. Their structures were elucidated by spectroscopic analysis. All isolated compounds were evaluated for their cytotoxicities against the KB and Vero cell lines and for antimalarial activities against the Plasmodium falciparum strains TM4 and K1 (a sensitive and a multi-drug-resistant strain, respectively). Compound 1 showed in vitro antimalarial activity against the TM4 strain, with an IC50 value of 19.3 ± 3.4 μM, and compound 2 demonstrated significant activity against the K1 strain, with an IC50 value of 10.8 ± 4.1 μM. Both compounds showed no discernible cytotoxicity to the Vero cell line at the concentration levels evaluated.

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