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  2. Design, Synthesis and Biological Evaluation of Novel Phosphorylated Abacavir Derivatives as Antiviral Agents Against Newcastle Disease Virus Infection in Chicken

Design, Synthesis and Biological Evaluation of Novel Phosphorylated Abacavir Derivatives as Antiviral Agents Against Newcastle Disease Virus Infection in Chicken

  • Appl Biochem Biotechnol. 2016 Sep;180(2):361-81. doi: 10.1007/s12010-016-2104-x.
Suresh K A 1 Kadiam C Venkata Subbaiah 1 Rayapu Lavanya 1 Kuruva Chandrasekhar 2 Naga Raju Chamarti 2 M Suresh Kumar 3 Rajendra Wudayagiri 4 Lokanatha Valluru 5
Affiliations

Affiliations

  • 1 Department of Biotechnology, Dravidian University, Kuppam, 517426, India.
  • 2 Department of Chemistry, Sri Venkateswara University, Tirupati, 517502, India.
  • 3 Centre for Bioinformatics, Pondicherry University, Pondicherry, 605014, India.
  • 4 Department of Zoology, Sri Venkateswara University, Tirupati, 517502, India.
  • 5 Department of Biotechnology, Dravidian University, Kuppam, 517426, India. lokanath.valluru@gmail.com.
Abstract

Newcastle disease virus is the most devastating virus in poultry industry. It can eradicate the entire poultry flocks once infected. This study is aimed to investigate the Antiviral efficacy of novel phosphorylated analogues of the drug abacavir (ABC) against Newcastle disease virus (NDV). About 16 analogues of ABC were designed and docking was performed against fusion protein of NDV. Three compounds were identified and selected for synthesis and biological evaluation based on binding affinity and docking scores. The compounds were synthesized and characterized by IR, (1)H, (13)C, (31)P and CHN analysis and mass spectra. These compounds were tested for Antiviral efficacy against NDV-infected DF-1 cells. Compound ABC-1 had shown potent Antiviral activity as evidenced by significant reduction in plaque units and cytopathic effect. Therefore, ABC-1 was selected to test for NDV-infected chicken survival rate. Effective dose50 concentrations were determined for ABC-1. Antioxidant Enzyme levels in brain, liver and lung tissues were estimated. Superoxide dismutase and catalase were significantly raised and lipid peroxidation and HA titer levels were decreased upon treatment with 2 mg/kg body weight ABC-1. Histopathological modifications were also restored in the ABC-1-treated group. These findings demonstrated ABC-1 as a potential Antiviral agent against NDV in chicken.

Keywords

Abacavir; Antiviral activity; Chicken; Docking; Newcastle disease virus; Phosphorylation.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-124938
    Newcastle Disease Virus Inhibitor