2. Academic Validation
  3. Residue level and dissipation pattern of lepimectin in shallots using high-performance liquid chromatography coupled with photodiode array detection

Residue level and dissipation pattern of lepimectin in shallots using high-performance liquid chromatography coupled with photodiode array detection

  • Biomed Chromatogr. 2016 Nov;30(11):1835-1842. doi: 10.1002/bmc.3759.
Sung-Woo Kim 1,  Md Musfiqur Rahman 1,  A M Abd El-Aty 2 3,  Lieu T B Truong 1,  Jeong-Heui Choi 1,  Joon-Seong Park 4,  Mi-Ra Kim 5,  Ho-Chul Shin 6,  Jae-Han Shim 7
Affiliations

Affiliations

  • 1 Biotechnology Research Institute, College of Agriculture and Life Science, Chonnam National University, Yongbong-ro 77, Buk-gu, Gwangju, 500-757, Republic of Korea.
  • 2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, Republic of Korea. abdelaty44@hotmail.com, amabdelaty@konkuk.ac.kr.
  • 3 Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt. abdelaty44@hotmail.com, amabdelaty@konkuk.ac.kr.
  • 4 Bio Control Research Center, Jeonnam Bioindustry Foundation, 495, Im-myeon, Gokseong-gun, Jeollanam-do, 516-942, Republic of Korea.
  • 5 Research Planning and Management Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Chungcheongbuk-do, 28159, Republic of Korea.
  • 6 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, Republic of Korea.
  • 7 Biotechnology Research Institute, College of Agriculture and Life Science, Chonnam National University, Yongbong-ro 77, Buk-gu, Gwangju, 500-757, Republic of Korea. jhshim@chonnam.ac.kr.
PMID: 27187504 DOI: 10.1002/bmc.3759
Abstract

Lepimectin, as an emulsifiable concentrate, was sprayed on shallots at the recommended dose rate (10 mL/20 L) to determine its residue levels, dissipation pattern, pre-harvest residue limits (PHRLs), and health risk. Samples were randomly collected over 10 days, extracted with acetonitrile, purified using an amino solid-phase extraction (NH2 -SPE) cartridge and analyzed using a high-performance liquid chromatography-photodiode array detection method. Field-incurred samples were confirmed using ultra-performance liquid chromatography-tandem mass spectrometry. The linearity was excellent, with a determination coefficient (R2 ) of ≥0.9991. The recoveries at two spiking levels (0.2 and 1.0 mg/kg) ranged from 84.49 to 87.64% with relative standard deviations of ≤7.04%. The developed method was applied to field samples grown in separate greenhouses, one located in Naju and one in Muan, in the Republic of Korea. The dissipation pattern was described by first-order kinetics with half-lives of 1.9 (Naju) and 1.7 days (Muan). The PHRL curves indicated that, if the lepimectin residues are <0.18 (Naju) and <0.13 mg/kg (Muan) 5 days before harvest, the residue levels will be lower than the maximum residue limit (0.05 mg/kg) upon harvesting. The risk assessment data indicated that lepimectin is safe for use in the cultivation of shallots, with no risk of detrimental effects to the consumer.

Keywords

dissipation pattern; lepimectin; liquid chromatography; pre-harvest residue limit; shallots; tandem mass spectrometry.

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