Exploring host-pathogen interactions through genome wide protein microarray analysis

Sci Rep. 2016 Jun 15;6:27996. doi: 10.1038/srep27996.

Luigi Scietti ¹, Katia Sampieri ¹, Irene Pinzuti ¹, Erika Bartolini ¹, Barbara Benucci ¹, Alessia Liguori ¹, Andreas F Haag ¹, Paola Lo Surdo ¹, Werner Pansegrau ¹, Vincenzo Nardi-Dei ¹, Laura Santini ¹, Seguinde Arora ², Xavier Leber ², Simonetta Rindi ³, Silvana Savino ¹, Paolo Costantino ¹, Domenico Maione ¹, Marcello Merola ¹ ⁴, Pietro Speziale ³, Matthew J Bottomley ¹, Fabio Bagnoli ¹, Vega Masignani ¹, Mariagrazia Pizza ¹, Meike Scharenberg ², Jean-Marc Schlaeppi ², Mikkel Nissum ¹, Sabrina Liberatori ¹

Affiliations

1 GSK Vaccines, Via Fiorentina 1, 53100 Siena, Italy.
2 Novartis Institutes for Biomedical Research, Novartis Campus, 4056 Basel, Switzerland.
3 Department of Molecular Medicine, Unit of Biochemistry, Viale Taramelli 3/b, 27100 Pavia, Italy.
4 University of Naples "Federico II", Department of Biology, via Cinthia 4, 80126 Naples, Italy.

PMID: 27302108 DOI: 10.1038/srep27996

Abstract

During Bacterial pathogenesis extensive contacts between the human and the Bacterial extracellular proteomes take place. The identification of novel host-pathogen interactions by standard methods using a case-by-case approach is laborious and time consuming. To overcome this limitation, we took advantage of large libraries of human and Bacterial recombinant proteins. We applied a large-scale protein microarray-based screening on two important human pathogens using two different approaches: (I) 75 human extracellular proteins were tested on 159 spotted Staphylococcus aureus recombinant proteins and (II) Neisseria meningitidis adhesin (NadA), an important vaccine component against serogroup B meningococcus, was screened against ≈2300 spotted human recombinant proteins. The approach presented here allowed the identification of the interaction between the S. aureus immune evasion protein FLIPr (formyl-peptide receptor like-1 inhibitory protein) and the human complement component C1q, key players of the offense-defense fighting; and of the interaction between meningococcal NadA and human LOX-1 (low-density oxidized lipoprotein receptor), an endothelial receptor. The novel interactions between Bacterial and human extracellular proteins here presented might provide a better understanding of the molecular events underlying S. aureus and N. meningitidis pathogenesis.

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