2. Academic Validation
  3. Azadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo

Azadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo

  • Bioorg Med Chem Lett. 2016 Aug 1;26(15):3719-24. doi: 10.1016/j.bmcl.2016.05.076.
Priyanka Kushwaha 1,  Vikram Khedgikar 1,  Saikat Haldar 2,  Jyoti Gautam 1,  Fayaj A Mulani 2,  Hirekodathakallu V Thulasiram 3,  Ritu Trivedi 4
Affiliations

Affiliations

  • 1 CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India.
  • 2 Chemical Biology Unit, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, India.
  • 3 Chemical Biology Unit, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, India; CSIR-Institute of Genomics and Integrative Biology, Mall Road, New Delhi 110007, India. Electronic address: hv.thulasiram@ncl.res.in.
  • 4 CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India. Electronic address: ritu_trivedi@cdri.res.in.
PMID: 27317644 DOI: 10.1016/j.bmcl.2016.05.076
Abstract

Terpenoids were isolated using chromatographic purification through solvent purification technique and identified as Azadirone (1), Epoxyazadiradione (2) Azadiradione (3) Gedunin (4) Nimbin (5) Salannin (6) Azadirachtin A (7) and Azadirachtin B (8) from Azadirachta indica. Out of eight compounds, only three compounds had osteogenic activity and enhanced osteoblast proliferation, differentiation and mineralization in osteoblast cells. Active compounds stimulated osteogenic genes ALP, RunX-2 and OCN expressions in vitro, but Azadirachtin A had a maximum ability to stimulate osteoblast differentiation and mineralization compared to other two active compounds. For in vivo study, Azadirachtin A injected subcutaneously in pups, which enhanced osteogenic gene expressions and promoted bone formation rate significantly. Here, we conclude that active compounds of Azadirachta indica have osteogenic activity and Azadirachtin A has a beneficial effects on bone.

Keywords

ALP and mineralization assay; Balb/c mice pups; Calvarial osteoblast cells; Gene expressions.

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