Azadirachtin B

Name: Azadirachtin B
Brand: MedChemExpress
SKU: HY-133108
Rating: 4.5 (1 reviews)

Cat. No.: HY-133108 Purity: 99.17%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Azadirachtin B is an limonoid isolated from seed kernels of Azadirachta indica. Azadirachtin B increases alkaline phosphatase (ALP) activity and stimulates osteoblast differentiation. Azadirachtin B is active against the Epstein-Barr virus early antigen (EBV-EA). Azadirachtin B has insecticidal, nematocidal, anticancer, anti-inflammatory, antiviral and osteogenic properties.

For research use only. We do not sell to patients.

Azadirachtin B Chemical Structure

CAS No. : 106500-25-8

Size	Price	Stock	Quantity
1 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 660	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1100	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Plutella xylostella^[1]
Alkaline phosphatase (ALP)^[2]
Epstein-Barr virus early antigen (EBV-EA)^[3]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 20 μM Compound: 21	Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 21381696]
AZ-521 cell line	IC₅₀	> 20 μM Compound: 21	Cytotoxicity against human AZ-521 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human AZ-521 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 21381696]
HL-60	IC₅₀	> 20 μM Compound: 21	Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 21381696]
SK-BR-3	IC₅₀	> 20 μM Compound: 21	Cytotoxicity against human SK-BR-3 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human SK-BR-3 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 21381696]

In Vitro

Azadirachtin B (1 pM-100 µM; 48 hours; Osteoblast cells) treatment shows highest proliferation at 10 nM and 100 pM concentrations in osteoblast cells^[1].
Azadirachtin B increases expression of RunX-2 ∼2.5 fold at 10 nM concentration, ALP expression ∼2.8 fold at 10 nM and 100 pM concentration and OCN expression ∼2.5 folds at 10 nM as compared with control^[1].
Azadirachtin B (Compound 4) exhibits toxicity to the diamondback moth (Plutella xylostella) with an LD₅₀ of 4.85-1.06 µg/g body weight, in 92 h^[2].
Azadirachtin B (compound 21) exhibits moderate or potent inhibitory effects (IC50 value of 384 mol ratio/32 pmol TPA) against the Epstein-Barr virus early antigen (EBV-EA) activation induced by tetradecanoylphorbol-13-acetate (TPA)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Osteoblast cells
Concentration:	1 pM, 100 pM, 10 nM, 1 µM, 100 µM
Incubation Time:	48 hours
Result:	Showed highest proliferation at 10 nM and 100 pM concentrations in osteoblast cells.

In Vivo

On evaluation of Azadirachtin B (compound 21; oral administration) for its anti-tumor-initiating activity on the two-stage carcinogenesis of mouse skin tumor induced by peroxynitrite (ONOO-; PN) as an initiator and TPA as a promoter, this exhibited marked inhibitory activity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

662.68

Formula

C₃₃H₄₂O₁₄

CAS No.

106500-25-8

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1([C@@]([C@H]2C(OC)=O)([H])[C@]([C@H](C[C@H]3OC(/C(C)=C/C)=O)O)(CO2)[C@@]4([H])[C@](OC[C@@]43C(OC)=O)([H])[C@H]1O)[C@]([C@@H](O[C@]5([H])OC=C6)C[C@H]7[C@]56O)(O8)[C@]78C

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (75.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5090 mL	7.5451 mL	15.0902 mL
5 mM	0.3018 mL	1.5090 mL	3.0180 mL
10 mM	0.1509 mL	0.7545 mL	1.5090 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.17%

Select Batch:

Data Sheet (283 KB) SDS (251 KB)

COA (262 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Kushwaha P, et al. Azadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo. Bioorg Med Chem Lett. 2016 Aug 1;26(15):3719-24. [Content Brief]

[2]. Kanokmedhakul S, et al. Azadirachtin derivatives from seed kernels of Azadirachta excelsa. J Nat Prod. 2005 Jul;68(7):1047-50. [Content Brief]

[3]. Akihisa T, et al. Melanogenesis inhibitory, anti-inflammatory, and chemopreventive effects of limonoids from the seeds of Azadirachta indicia A. Juss. (neem). J Oleo Sci. 2009;58(11):581-94. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5090 mL	7.5451 mL	15.0902 mL	37.7256 mL
	5 mM	0.3018 mL	1.5090 mL	3.0180 mL	7.5451 mL
	10 mM	0.1509 mL	0.7545 mL	1.5090 mL	3.7726 mL
	15 mM	0.1006 mL	0.5030 mL	1.0060 mL	2.5150 mL
	20 mM	0.0755 mL	0.3773 mL	0.7545 mL	1.8863 mL
	25 mM	0.0604 mL	0.3018 mL	0.6036 mL	1.5090 mL
	30 mM	0.0503 mL	0.2515 mL	0.5030 mL	1.2575 mL
	40 mM	0.0377 mL	0.1886 mL	0.3773 mL	0.9431 mL
	50 mM	0.0302 mL	0.1509 mL	0.3018 mL	0.7545 mL
	60 mM	0.0252 mL	0.1258 mL	0.2515 mL	0.6288 mL

Azadirachtin B Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Azadirachtin B

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