2. Academic Validation
  3. SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability

SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability

  • Nat Commun. 2016 Jul 20;7:12235. doi: 10.1038/ncomms12235.
Lei Li 1,  Lan Shi 1,  Shangda Yang 2,  Ruorong Yan 1,  Di Zhang 1,  Jianguo Yang 1,  Lin He 1,  Wanjin Li 1,  Xia Yi 1,  Luyang Sun 1,  Jing Liang 1,  Zhongyi Cheng 3,  Lei Shi 2,  Yongfeng Shang 1 2,  Wenhua Yu 1
Affiliations

Affiliations

  • 1 Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
  • 2 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
  • 3 Jingjie PTM BioLab Co. Ltd., Hangzhou Economic and Technological Development Area, Hangzhou 310018, China.
PMID: 27436229 DOI: 10.1038/ncomms12235
Abstract

Although SIRT7 is a member of Sirtuin family proteins that are described as NAD(+)-dependent class III histone deacetylases, the intrinsic enzymatic activity of this Sirtuin protein remains to be investigated and the cellular function of SIRT7 remains to be explored. Here we report that SIRT7 is an NAD(+)-dependent histone desuccinylase. We show that SIRT7 is recruited to DNA double-strand breaks (DSBs) in a PARP1-dependent manner and catalyses desuccinylation of H3K122 therein, thereby promoting chromatin condensation and DSB repair. We demonstrate that depletion of SIRT7 impairs chromatin compaction during DNA-damage response and sensitizes cells to genotoxic stresses. Our study indicates SIRT7 is a histone desuccinylase, providing a molecular basis for the understanding of epigenetic regulation by this Sirtuin protein. Our experiments reveal that SIRT7-catalysed H3K122 desuccinylation is critically implemented in DNA-damage response and cell survival, providing a mechanistic insight into the cellular function of SIRT7.

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