2. Academic Validation
  3. Chx10 Consolidates V2a Interneuron Identity through Two Distinct Gene Repression Modes

Chx10 Consolidates V2a Interneuron Identity through Two Distinct Gene Repression Modes

  • Cell Rep. 2016 Aug 9;16(6):1642-1652. doi: 10.1016/j.celrep.2016.06.100.
Yoanne M Clovis 1,  So Yeon Seo 2,  Ji-Sun Kwon 1,  Jennifer C Rhee 2,  Sujeong Yeo 2,  Jae W Lee 1,  Seunghee Lee 3,  Soo-Kyung Lee 4
Affiliations

Affiliations

  • 1 Pediatric Neuroscience Research Program, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health and Science University, Portland, OR 97239, USA.
  • 2 College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Korea.
  • 3 College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Korea. Electronic address: leeseung@snu.ac.kr.
  • 4 Pediatric Neuroscience Research Program, Papé Family Pediatric Research Institute, Department of Pediatrics, Oregon Health and Science University, Portland, OR 97239, USA; Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA. Electronic address: leesoo@ohsu.edu.
PMID: 27477290 DOI: 10.1016/j.celrep.2016.06.100
Abstract

During development, two cell types born from closely related progenitor pools often express identical transcriptional regulators despite their completely distinct characteristics. This phenomenon implies the need for a mechanism that operates to segregate the identities of the two cell types throughout differentiation after initial fate commitment. To understand this mechanism, we investigated the fate specification of spinal V2a interneurons, which share important developmental genes with motor neurons (MNs). We demonstrate that the paired homeodomain factor Chx10 functions as a critical determinant for V2a fate and is required to consolidate V2a identity in postmitotic neurons. Chx10 actively promotes V2a fate, downstream of the LIM-homeodomain factor Lhx3, while concomitantly suppressing the MN developmental program by preventing the MN-specific transcription complex from binding and activating MN genes. This dual activity enables Chx10 to effectively separate the V2a and MN pathways. Our study uncovers a widely applicable gene regulatory principle for segregating related cell fates.

Keywords

Chx10; Lhx3; Sox14; V2a interneurons; Vsx2; development; motor neurons; spinal cord; transcription factor.

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