2. Academic Validation
  3. The role of Ca(2+) mediated signaling pathways on the effect of taurine against Streptococcus uberis infection

The role of Ca(2+) mediated signaling pathways on the effect of taurine against Streptococcus uberis infection

  • Vet Microbiol. 2016 Aug 30;192:26-33. doi: 10.1016/j.vetmic.2016.06.008.
Bin Dai 1,  Jinqiu Zhang 2,  Ming Liu 1,  Jinye Lu 3,  Yuanshu Zhang 1,  Yuanyuan Xu 1,  Jinfeng Miao 4,  Yulong Yin 5
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
  • 2 National Research Center for Veterinary Vaccine Engineering and Technology of China, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
  • 3 Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China.
  • 4 College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: mjf171647@126.com.
  • 5 Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center for Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center for Animal & Poultry Science, Key Laboratory of Agroecology in Subtropical Region, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central China, Ministry of Agriculture, Changsha 410125, China.
PMID: 27527761 DOI: 10.1016/j.vetmic.2016.06.008
Abstract

To provide insight into the mechanisms of taurine attenuation of pro-inflammatory response in mouse mammary epithelial cell line (EpH4-Ev, purchased by ATCC, USA) after Streptococcus uberis (S. uberis, 0140J) challenge, we infected MECs with S. uberis (2.5×10(7)cfumL(-1), MOI=10) for 3h and quantified changes in TLR-2 and calcium (CA(2+)) mediated signaling pathways. The results indicate that S. uberis Infection significantly increases the expression of TLR-2, intracellular CA(2+) levels, PLC-γ1 and PKC-α, the activities of transcription factors NF-κB and NFAT, and related cytokines (TNF-α, IL-1β, IL-6, G-CSF, IL-2, KC, IL-15, FasL, MCP-1, and LIX) in culture supernatants. Taurine administration downregulated all these indices, the activities of NF-κB and NFAT. Cytokine secretions were similar using special PKC Inhibitor Go 6983 and NFAT inhibitor VIVIT. Our data indicate that S. uberis Infection induces pro-inflammatory response of MECs through a TLR-2 mediated signaling pathway. In addition, taurine can prevent MEC damage by affecting both PLC-γ1-Ca(2+)-PKC-α-NF-κB and PLC-γ1-Ca(2+)-NFATs signaling pathways. This is the first report to demonstrate the mechanisms of taurine attenuated pro-inflammatory response in MECs after S. uberis challenge.

Keywords

Ca(2+); Mouse mammary epithelial cell; Streptococcus uberis; Taurine.

Figures
Products
  • Cat. No.
    Product Name
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  • HY-13689
    99.32%, PKC Inhibitor
    PKC