2. Academic Validation
  3. Identification of 2'-5'-Oligoadenylate Synthetase-Like Gene in Goose: Gene Structure, Expression Patterns, and Antiviral Activity Against Newcastle Disease Virus

Identification of 2'-5'-Oligoadenylate Synthetase-Like Gene in Goose: Gene Structure, Expression Patterns, and Antiviral Activity Against Newcastle Disease Virus

  • J Interferon Cytokine Res. 2016 Sep;36(9):563-72. doi: 10.1089/jir.2015.0167.
Chao Yang 1,  Fei Liu 2,  Shun Chen 1 2 3,  Mingshu Wang 1 2 3,  Renyong Jia 1 2 3,  Dekang Zhu 2 3,  Mafeng Liu 1,  Kunfeng Sun 1 2 3,  Qiao Yang 1 2 3,  Ying Wu 1 2 3,  Xiaoyue Chen 2 3,  Anchun Cheng 1 2 3
Affiliations

Affiliations

  • 1 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .
  • 2 2 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University , Chengdu, China .
  • 3 3 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .
PMID: 27576097 DOI: 10.1089/jir.2015.0167
Abstract

2'-5'-oligoadenylate synthetase-like (OASL) is a kind of Antiviral protein induced by interferons (IFNs), which plays an important role in the IFNs-mediated Antiviral signaling pathway. In this study, we cloned and identified OASL in the Chinese goose for the first time. Goose 2'-5'-oligoadenylate synthetase-like (goOASL), including an ORF of 1527bp, encoding a protein of 508 Amino acids. GoOASL protein contains 3 conserved motifs: nucleotidyltransferase (NTase) domain, 2'-5'-oligoadenylate synthetase (OAS) domain, and 2 ubiquitin-like (UBL) repeats. The tissue distribution profile of goOASL in 2-week-old gosling and adult goose were identified by Real-Time quantitative PCR, which revealed that the highest level of goOASL mRNA transcription was detected in the blood of adult goose and gosling. The mRNA transcription level of goOASL was upregulated in all tested tissues of duck Tembusu virus (DTMUV)-infected 3-day-old goslings, compared with control groups. Furthermore, using the stimulus Poly(I: C), ODN2006, R848, and lipopolysaccharide (LPS) as well as the viral pathogens DTMUV, H9N2 avian Influenza Virus (AIV), and gosling plague virus (GPV) to treat goose peripheral blood mononuclear cells (PBMCs) for 6 h, goOASL transcripts level was significantly upregulated in all treated groups. To further investigate the Antiviral activity of goOASL, pcDNA3.1(+)-goOASL-His plasmid was constructed, and goOASL was expressed by the goose embryo fibroblast cells (GEFs) transfected with pcDNA3.1(+)-goOASL-His. Our research data suggested that Newcastle disease virus (NDV) replication (viral copies and viral titer) in GEFs was significantly reduced by the overexpression of goOASL protein. These data were meaningful for the Antiviral immunity research of goose and shed LIGHT on the future prevention of NDV in fowl.

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