1. Academic Validation
  2. D-Phenylalanine inhibits biofilm development of a marine microbe, Pseudoalteromonas sp. SC2014

D-Phenylalanine inhibits biofilm development of a marine microbe, Pseudoalteromonas sp. SC2014

  • FEMS Microbiol Lett. 2016 Sep;363(18):fnw198. doi: 10.1093/femsle/fnw198.
Ee Li 1 Jiajia Wu 2 Peng Wang 2 Dun Zhang 3
Affiliations

Affiliations

  • 1 Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China University of Chinese Academy of Sciences, 19 (Jia) Yuquan Road, Beijing 100039, China.
  • 2 Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China.
  • 3 Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China zhangdun@qdio.ac.cn.
Abstract

D-Amino acids have been reported to be able to inhibit biofilm formation or disperse existing biofilms of many microbes; in some cases this is due to growth inhibition as an unspecific effect. In this work, six different D-amino acids were tested for their inhibitory effects on biofilm development and Bacterial growth of Pseudoalteromonas sp. SC2014, a marine microbe involved in microbiologically influenced corrosion (MIC). Experimental results indicated that D-phenylalanine (D-Phe) inhibited biofilm formation effectively at concentrations that did not affect cell growth, whereas the other D-amino acids either showed little effect or inhibited biofilm formation while inhibiting Bacterial growth. Further studies found that D-Phe could inhibit Bacterial accumulation on the surface of 316L stainless steel, and prevent bacteria from forming a multilayer biofilm. It was also suggested that D-Phe could promote the disassembly of an established multilayer biofilm but have little effect on the remaining monolayer adherent cells. For the first time, it was found that a D-amino acid could effectively inhibit biofilm formation of an MIC-involved microbe. This might supply a new insight into how MIC could be mitigated.

Keywords

D-phenylalanine; Pseudoalteromonas sp. SC2014; biofilm; microbiologically influenced corrosion.

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