2. Academic Validation
  3. Anti-proliferation effects, efficacy of cyasterone in vitro and in vivo and its mechanism

Anti-proliferation effects, efficacy of cyasterone in vitro and in vivo and its mechanism

  • Biomed Pharmacother. 2016 Dec;84:330-339. doi: 10.1016/j.biopha.2016.09.041.
XinGang Lu 1,  HongFu Qiu 2,  Liu Yang 3,  JieYing Zhang 4,  ShuJie Ma 5,  Lan Zhen 6
Affiliations

Affiliations

  • 1 Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University School of Medicine, Shanghai, 200040, PR China. Electronic address: lxg1082@gmail.com.
  • 2 Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University School of Medicine, Shanghai, 200040, PR China.
  • 3 Department of Oncology, BaoShan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 201999, PR China.
  • 4 Department of Traditional Chinese Medicine, PuTuo Hospital of Traditional Chinese Medicine, Shanghai, 200063, PR China.
  • 5 Department of Rehabilitation, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, PR China.
  • 6 Department of Traditional Chinese Medicine, RuiJin Hospital, JiaoTong University School of Medicine, Shanghai, 200025, PR China, PR China.
PMID: 27668532 DOI: 10.1016/j.biopha.2016.09.041
Abstract

Cyasterone was demonstrated potential inhibition effect in mouse skin carcinoma cells in published report. However, the molecular mechanisms of the cyasterone on cells remain unknown. Herein, we investigated the effects of cyasterone-induced Apoptosis in A549 and MGC823 cells in vitro. MTT assay showed that cyasterone caused a significantly decreasing of the proliferation of A549 and MGC823 cells in a time-and dose-dependent manner with IC50 values of 38.50±3.73μg/mL on A549 cells and 32.96±1.24μg/mL on MGC823 cells at 48h, respectively. Hoechst staining and TUNEL staining results indicated the quintessential Apoptosis features in immunofluorescence image. Apoptosis and cell cycle were determined by flow cytometry. Cyasterone treatment triggered inhibition of epidermal growth factor receptor- phosphatidylinositol 3 kinase/protein kinase B (EGFR-AKT) signaling pathways and activation of P38 pathways. Furthermore, cyasterone inhibited MGC823 cells xenografted tumor growth in vivo with few changes in body weights. In conclusion, our findings provide the evidence that cyasterone inhibits growth of A549 and MGC823 cells, via regulating EGFR signaling pathway. Our results indicated that cyasterone, a natural EGFR inhibitor, maybe a promising anti-cancer agent.

Keywords

Apoptosis; Cell cycle arrest; Cyasterone; EGFR signaling pathway.

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