2. Academic Validation
  3. TIM29 is a subunit of the human carrier translocase required for protein transport

TIM29 is a subunit of the human carrier translocase required for protein transport

  • FEBS Lett. 2016 Dec;590(23):4147-4158. doi: 10.1002/1873-3468.12450.
Sylvie Callegari 1,  Frank Richter 1,  Katarzyna Chojnacka 2,  Daniel C Jans 3 4,  Isotta Lorenzi 1,  David Pacheu-Grau 1,  Stefan Jakobs 3 4,  Christof Lenz 5 6,  Henning Urlaub 5 6,  Jan Dudek 1,  Agnieszka Chacinska 2,  Peter Rehling 1 7
Affiliations

Affiliations

  • 1 Department of Cellular Biochemistry, University Medical Center Göttingen, Germany.
  • 2 International Institute of Molecular and Cell Biology, Warsaw, Poland.
  • 3 Department of NanoBiophotonics, Mitochondrial Structure and Dynamics Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • 4 Department of Neurology, University Medical Center Göttingen, Germany.
  • 5 Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • 6 Bioanalytics, Institute for Clinical Chemistry, University Medical Center Göttingen, Germany.
  • 7 MaxPlanck Institute for Biophysical Chemistry, Göttingen, Germany.
PMID: 27718247 DOI: 10.1002/1873-3468.12450
Abstract

Hydrophobic inner mitochondrial membrane proteins with internal targeting signals, such as the metabolite carriers, use the carrier translocase (TIM22 complex) for transport into the inner membrane. Defects in this transport pathway have been associated with neurodegenerative disorders. While the TIM22 complex is well studied in baker's yeast, very little is known about the mammalian TIM22 complex. Using immunoprecipitation, we purified the human carrier translocase and identified a mitochondrial inner membrane protein TIM29 as a novel component, specific to metazoa. We show that TIM29 is a constituent of the 440 kDa TIM22 complex and interacts with oxidized TIM22. Our analyses demonstrate that TIM29 is required for the structural integrity of the TIM22 complex and for import of substrate proteins by the carrier translocase.

Keywords

TIM22; TIM29; carrier translocase; membrane protein; mitochondria; protein translocation.

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