Biosynthetic approaches to creating bioactive fungal metabolites: Pathway engineering and activation of secondary metabolism

The diversity of Natural Products is greater than that of combinatorial chemistry compounds and is similar to that of drugs. Compounds rich in sp³ carbons, such as Natural Products, typically exhibit high structural complexity and high specificity to molecular targets. Microorganisms can synthesize such sp³ carbon-rich compounds and can be used as excellent factories for making bioactive compounds. Here, we mainly focus on pathway engineering of two sp³ carbon-rich bioactive Indole Alkaloids, fumitremorgin C and terpendole E. We also demonstrate the importance of activation of secondary metabolism by focusing on tenuazonic acid, a bioactive tetramic acid compound, as an example.

Fumitremorgin C; Indole-diterpene; Mycotoxin; Nonribosomal peptide; Pathway engineering; Terpendole E.