2. Academic Validation
  3. Angiogenesis Inhibitors and Anti-Inflammatory Agents from Phoma sp. NTOU4195

Angiogenesis Inhibitors and Anti-Inflammatory Agents from Phoma sp. NTOU4195

  • J Nat Prod. 2016 Dec 23;79(12):2983-2990. doi: 10.1021/acs.jnatprod.6b00407.
Ming-Shian Lee,  Shih-Wei Wang 1,  Guei-Jane Wang 2 3 4,  Ka-Lai Pang 5,  Ching-Kuo Lee,  Yueh-Hsiung Kuo 6 7,  Hyo-Jung Cha 5,  Ruo-Kai Lin,  Tzong-Huei Lee 8
Affiliations

Affiliations

  • 1 Department of Medicine, Mackay Medical College , New Taipei City 25245, Taiwan.
  • 2 School of Medicine, Graduate Institute of Clinical Medical Science, China Medical University , Taichung 40402, Taiwan.
  • 3 Department of Medical Research, China Medical University Hospital , Taichung 40447, Taiwan.
  • 4 Department of Health and Nutrition Biotechnology, Asia University , Taichung 41354, Taiwan.
  • 5 Institute of Marine Biology and Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung 20224, Taiwan.
  • 6 Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University , Taichung 40447, Taiwan.
  • 7 Department of Biotechnology, Asia University , Taichung 41354, Taiwan.
  • 8 Institute of Fisheries Science, National Taiwan University , Taipei 10617, Taiwan.
PMID: 27976895 DOI: 10.1021/acs.jnatprod.6b00407
Abstract

Seven new polyketides, phomaketides A-E (1-5) and pseurotins A3 (6) and G (7), along with the known compounds FR-111142, pseurotins A, A1, A2, D, and F2, 14-norpseurotin A, α-carbonylcarbene, tyrosol, cyclo(-l-Pro-l-Leu), and cyclo(-l-Pro-l-Phe), were purified from the fermentation broth and mycelium of the endophytic Fungal strain Phoma sp. NTOU4195 isolated from the marine red alga Pterocladiella capillacea. The structures were established through interpretation of spectroscopic data. The antiangiogenic and anti-inflammatory effects of 1-7 and related analogues were evaluated using human endothelial progenitor cells (EPCs) and lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells, respectively. Of the compounds tested, compound 1 exhibited the most potent antiangiogenic activity by suppressing the tube formation of EPCs with an IC50 of 8.1 μM, and compound 3 showed the most selective inhibitory activity of LPS-induced NO production in RAW264.7 macrophages with an IC50 value of 8.8 μM.

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