2. Academic Validation
  3. Natural NO inhibitors from the leaves of Callicarpa kwangtungensis: Structures, activities, and interactions with iNOS

Natural NO inhibitors from the leaves of Callicarpa kwangtungensis: Structures, activities, and interactions with iNOS

  • Bioorg Med Chem Lett. 2017 Feb 1;27(3):670-674. doi: 10.1016/j.bmcl.2016.11.044.
Shen Li 1,  Xiaocong Sun 1,  Yang Li 1,  Feng Liu 1,  Jun Ma 1,  Ling Tong 2,  Guochen Su 1,  Jing Xu 3,  Yasushi Ohizumi 4,  Dongho Lee 5,  Yuanqiang Guo 6
Affiliations

Affiliations

  • 1 State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
  • 2 State Key Laboratory of Core Technology in Innovative Chinese Medicine, Pharmaceutical Analysis Institute, Tasly Academy, Tianjin 300410, People's Republic of China.
  • 3 State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address: xujing611@nankai.edu.cn.
  • 4 Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
  • 5 Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
  • 6 State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address: victgyq@nankai.edu.cn.
PMID: 27989668 DOI: 10.1016/j.bmcl.2016.11.044
Abstract

A phytochemical investigation to obtain new NO inhibitors resulted in the isolation of a new diterpenoid with a rare 9,10-seco-abietane skeleton (1) and twelve known Terpenoids (2-13) from Callicarpa kwangtungensis. Their structures were elucidated on the basis of extensive 1D and 2D NMR spectroscopic data analyses, and the absolute configuration of compound 1 was established by comparison of the calculated and experimental electronic circular dichroism (ECD) spectra. The inhibitory activities on lipopolysaccharide-induced NO production in murine microglial BV-2 cells of these Terpenoids were evaluated, and all of the compounds showed inhibitory effects. The following molecular docking studies showed interactions of the bioactive compounds with the iNOS protein.

Keywords

9,10-seco-Abietane diterpenoid; Callicarpa kwangtungensis; Molecular docking; NO inhibitory activities; Terpenoids; iNOS.

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