2. Academic Validation
  3. The amino acid transporter, SLC1A3, is plasma membrane-localised in adipocytes and its activity is insensitive to insulin

The amino acid transporter, SLC1A3, is plasma membrane-localised in adipocytes and its activity is insensitive to insulin

  • FEBS Lett. 2017 Jan;591(2):322-330. doi: 10.1002/1873-3468.12549.
James R Krycer 1,  Daniel J Fazakerley 1,  Rosemary J Cater 2,  Kristen C Thomas 1,  Sheyda Naghiloo 1,  James G Burchfield 1,  Sean J Humphrey 1,  Robert J Vandenberg 2,  Renae M Ryan 2,  David E James 1 3
Affiliations

Affiliations

  • 1 School of Life and Environmental Sciences and Charles Perkins Centre, The University of Sydney, NSW, Australia.
  • 2 Discipline of Pharmacology, Sydney Medical School, The University of Sydney, NSW, Australia.
  • 3 Sydney Medical School, The University of Sydney, NSW, Australia.
PMID: 28032905 DOI: 10.1002/1873-3468.12549
Abstract

The hormone Insulin coordinates the catabolism of nutrients by protein phosphorylation. Phosphoproteomic analysis identified insulin-responsive phosphorylation of the Glu/Asp transporter SLC1A3/EAAT1 in adipocytes. The role of SLC1A3 in adipocytes is not well-understood. We show that SLC1A3 is localised to the plasma membrane and the major regulator of acidic amino acid uptake in adipocytes. However, its localisation and activity were unaffected by Insulin or mutation of the insulin-regulated phosphosite. The latter was also observed using a heterologous expression system in Xenopus laevis oocytes. Thus, SLC1A3 maintains a constant import of acidic Amino acids independently of nutritional status in adipocytes.

Keywords

SLC1A3/EAAT1; adipocyte; insulin.

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