2. Academic Validation
  3. Application of modified yeast surface display technologies for non-Antibody protein engineering

Application of modified yeast surface display technologies for non-Antibody protein engineering

  • Microbiol Res. 2017 Mar:196:118-128. doi: 10.1016/j.micres.2016.12.002.
Meng Mei 1,  Yu Zhou 1,  Wenfang Peng 1,  Chan Yu 1,  Lixin Ma 1,  Guimin Zhang 2,  Li Yi 3
Affiliations

Affiliations

  • 1 Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China.
  • 2 Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China. Electronic address: zhangguimin6@hotmail.com.
  • 3 Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China. Electronic address: liyi@hubu.edu.cn.
PMID: 28164786 DOI: 10.1016/j.micres.2016.12.002
Abstract

Yeast surface display (YSD) system has been widely used in protein engineering since it was established 20 years ago. Combined with fluorescence-activated cell sorting (FACS) technology and directed evolution, YSD has been proven of its extraordinary effectiveness for molecular engineering of various target proteins, especially for Antibodies. Recently, a few remarkable efforts were exploited to modify the original Aga1-Aga2 YSD for the non-antibody protein engineering with successful outcomes, expanding its application on oxidase, Class II major histocompatibility complex (MHC-II), Protease, sortase, lipoic acid Ligase etc. Here, the methodologies of these optimized Aga1-Aga2 YSD technologies were introduced, and the recent progress of non-antibody protein engineering using these methods was summarized.

Keywords

Directed evolution; FACS; Non-antibody protein engineering; Yeast surface display.

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