2. Academic Validation
  3. Robust, universal biomarker assay to detect senescent cells in biological specimens

Robust, universal biomarker assay to detect senescent cells in biological specimens

  • Aging Cell. 2017 Feb;16(1):192-197. doi: 10.1111/acel.12545.
Konstantinos Evangelou 1,  Nikolaos Lougiakis 2,  Sophia V Rizou 1,  Athanassios Kotsinas 1,  Dimitris Kletsas 3,  Daniel Muñoz-Espín 4,  Nikolaos G Kastrinakis 1,  Nicole Pouli 2,  Panagiotis Marakos 2,  Paul Townsend 5,  Manuel Serrano 4,  Jiri Bartek 6 7,  Vassilis G Gorgoulis 1 5 8
Affiliations

Affiliations

  • 1 Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
  • 2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece.
  • 3 Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications, National Centre for Scientific Research 'Demokritos', Athens, Greece.
  • 4 Tumor Suppression Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
  • 5 Molecular and Clinical Cancer Sciences, Manchester Cancer Research Centre, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.
  • 6 Danish Cancer Society Research Center, Copenhagen, Denmark.
  • 7 Science For Life Laboratory, Division of Translational Medicine and Chemical Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
  • 8 Biomedical Research Foundation, Academy of Athens, Athens, Greece.
PMID: 28165661 DOI: 10.1111/acel.12545
Abstract

Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from Cancer research to gerontology.

Keywords

Sudan Black B; aging; biotin‐linked compounds; immunohistochemistry; senescence.

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