2. Academic Validation
  3. Hormonemate Derivatives from Dothiora sp., an Endophytic Fungus

Hormonemate Derivatives from Dothiora sp., an Endophytic Fungus

  • J Nat Prod. 2017 Apr 28;80(4):845-853. doi: 10.1021/acs.jnatprod.6b00680.
Mercedes Pérez-Bonilla 1,  Víctor González-Menéndez 1,  Ignacio Pérez-Victoria 1,  Nuria de Pedro 1,  Jesús Martín 1,  Joaquín Molero-Mesa 2,  Manuel Casares-Porcel 2,  María Reyes González-Tejero 2,  Francisca Vicente 1,  Olga Genilloud 1,  José R Tormo 1,  Fernando Reyes 1
Affiliations

Affiliations

  • 1 Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía , Parque Tecnológico Ciencias de la Salud, Avda. del Conocimiento 34, 18016 Armilla, Granada, Spain.
  • 2 Departamento de Botánica, Facultad de Farmacia, Universidad de Granada , 18071 Granada, Spain.
PMID: 28277681 DOI: 10.1021/acs.jnatprod.6b00680
Abstract

A search for cytotoxic agents from cultures of the endophytic fungus Dothiora sp., isolated from the endemic plant Launaea arborescens, led to the isolation of six new compounds structurally related to hormonemate, with moderate cytotoxic activity against different Cancer cell lines. By using a bioassay-guided fractionation approach, hormonemates A-D (1-4), hormonemate (5), and hormonemates E (6) and F (7) were obtained from the acetone extract of this fungus. Their structures were determined using a combination of HRMS, ESI-qTOF-MS/MS, 1D and 2D NMR experiments, and chemical degradation. The cytotoxic activities of these compounds were evaluated by microdilution colorimetric assays against human breast adenocarcinoma (MCF-7), human liver Cancer cells (HepG2), and pancreatic Cancer cells (MiaPaca_2). Most of the compounds displayed cytotoxic activity against this panel.

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