2. Academic Validation
  3. Clerodane Diterpenoid Glucosides from the Stems of Tinospora sinensis

Clerodane Diterpenoid Glucosides from the Stems of Tinospora sinensis

  • J Nat Prod. 2017 Apr 28;80(4):975-982. doi: 10.1021/acs.jnatprod.6b00976.
Huan Jiang 1,  Gui-Jie Zhang 2,  Yan-Fei Liu 3,  Heng-Shan Wang 1,  Dong Liang 1
Affiliations

Affiliations

  • 1 State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University , Guilin 541004, People's Republic of China.
  • 2 College of Pharmacy, Guilin Medical University , Guilin 541004, People's Republic of China.
  • 3 State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100050, People's Republic of China.
PMID: 28358196 DOI: 10.1021/acs.jnatprod.6b00976
Abstract

Ten new clerodane diterpenoid glucosides (1-10) and three known analogues (11-13) were isolated from an EtOAc extract of the stems of Tinospora sinensis. Spectroscopic analyses and chemical methods were used to elucidate the structures of these isolates. The absolute configurations of tinosinenosides A-C (1-3) were established by using experimental and calculated ECD data. Their cytotoxicity against the human epithelioid cervical carcinoma (HeLa) cell line and the nitric oxide production inhibitory activity of lipopolysaccharide-activated N9 microglial cells were tested. 1-Deacetyltinosposide A (12) exhibited mild cytotoxicity against HeLa cells, with an IC50 value of 8.35 ± 0.60 μM.

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