2. Academic Validation
  3. Preussilides A-F, Bicyclic Polyketides from the Endophytic Fungus Preussia similis with Antiproliferative Activity

Preussilides A-F, Bicyclic Polyketides from the Endophytic Fungus Preussia similis with Antiproliferative Activity

  • J Nat Prod. 2017 May 26;80(5):1531-1540. doi: 10.1021/acs.jnatprod.7b00064.
Sara R Noumeur 1 2 3,  Soleiman E Helaly 1 4,  Rolf Jansen 1,  Marcus Gereke 5,  Theresia E B Stradal 5,  Daoud Harzallah 2,  Marc Stadler 1
Affiliations

Affiliations

  • 1 Department of Microbial Drugs, Helmholtz Centre for Infection Research and German Centre for Infection Research (DZIF) , partner site Hannover/Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
  • 2 Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Sétif 1 Ferhat Abbas , 19000 Sétif, Algeria.
  • 3 Department of Microbiology-Biochemistry, Faculty of Natural and Life Sciences, University of Batna 2 , 05000 Batna, Algeria.
  • 4 Department of Chemistry, Faculty of Science, Aswan University , 81528 Aswan, Egypt.
  • 5 Department of Cell Biology, Helmholtz Centre for Infection Research , Inhoffenstrasse 7, 38124 Braunschweig, Germany.
PMID: 28398049 DOI: 10.1021/acs.jnatprod.7b00064
Abstract

Six novel bioactive bicyclic polyketides (1-6) were isolated from cultures of an endophytic fungus of the medicinal plant Globularia alypum collected in Batna, Algeria. The producer organism was identified as Preussia similis using morphological and molecular phylogenetic methods. The structures of metabolites 1-6, for which the trivial names preussilides A-F are proposed, were elucidated using a combination of spectral methods, including extensive 2D NMR spectroscopy, high-resolution mass spectrometry, and CD spectroscopy. Preussilides were tested for antimicrobial and antiproliferative effects, and, in particular, compounds 1 and 3 showed selective activities against eukaryotes. Subsequent studies on the influence of 1 and 3 on the morphology of human osteosarcoma cells (U2OS) suggest that these two polyketides might target an Enzyme involved in coordination of the cell division cycle. Hence, they might, for instance, affect timing or spindle assembly mechanisms, leading to defects in chromosome segregation and/or spindle geometry.

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